Protein Info for GFF2809 in Pseudomonas sp. DMC3

Annotation: Protein-glutamate methylesterase/protein-glutamine glutaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 PF00072: Response_reg" amino acids 124 to 233 (110 residues), 85.7 bits, see alignment E=5.1e-28 PF08281: Sigma70_r4_2" amino acids 253 to 302 (50 residues), 33 bits, see alignment 7.7e-12 PF00196: GerE" amino acids 259 to 312 (54 residues), 66 bits, see alignment 3.7e-22

Best Hits

KEGG orthology group: None (inferred from 56% identity to ppw:PputW619_0231)

Predicted SEED Role

"Nitrogen regulation protein NR(I)" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (329 amino acids)

>GFF2809 Protein-glutamate methylesterase/protein-glutamine glutaminase (Pseudomonas sp. DMC3)
MIRLGQATISLERREAFLDGRPLRMGGRAFEILSVLMQADGRIVSKDELINQVWPDIVVE
ENNLQVQISSLRKLLGEKEIIQTVARRGYRLLKDREPKLPVFNALASAPMVAQPPLVDTQ
SVPVFIVDDEACVRSALSRLLRAEDIEHRIFQSAEELLEADIANGPACLLLDVSLPETTG
LQLQAALNERGLPWPVIFMTGHGTIPMSVQAMKAGAVEFLTKPFDDDELLGILRSTRQYA
ATAFNQWQRVQHVRQLAAQLTPREREVLPLIVGGLSNKQIANQLGTSEVTAKVHRKHIMA
KMQTRSLVNLVELYGLISAAPSPGPSSLS