Protein Info for GFF2792 in Variovorax sp. SCN45

Annotation: Chaperone protein ClpB (ATP-dependent unfoldase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 873 TIGR03346: ATP-dependent chaperone protein ClpB" amino acids 6 to 851 (846 residues), 1377.8 bits, see alignment E=0 PF02861: Clp_N" amino acids 17 to 67 (51 residues), 44.3 bits, see alignment 9.3e-15 amino acids 93 to 143 (51 residues), 31.4 bits, see alignment 1e-10 PF00004: AAA" amino acids 201 to 335 (135 residues), 47.9 bits, see alignment E=1.1e-15 amino acids 605 to 732 (128 residues), 39 bits, see alignment E=6.5e-13 PF17871: AAA_lid_9" amino acids 340 to 439 (100 residues), 119.7 bits, see alignment E=3.1e-38 PF07724: AAA_2" amino acids 599 to 761 (163 residues), 234 bits, see alignment E=6.1e-73 PF00158: Sigma54_activat" amino acids 602 to 721 (120 residues), 20.6 bits, see alignment E=1.8e-07 PF07728: AAA_5" amino acids 604 to 724 (121 residues), 47.4 bits, see alignment E=1.3e-15 PF10431: ClpB_D2-small" amino acids 768 to 848 (81 residues), 86.4 bits, see alignment E=6.3e-28

Best Hits

Swiss-Prot: 78% identical to CLPB_RALSO: Chaperone protein ClpB (clpB) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K03695, ATP-dependent Clp protease ATP-binding subunit ClpB (inferred from 85% identity to aaa:Acav_1267)

MetaCyc: 66% identical to protein disaggregase ClpB (Escherichia coli K-12 substr. MG1655)
RXN185E-10

Predicted SEED Role

"ClpB protein" in subsystem Protein chaperones or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (873 amino acids)

>GFF2792 Chaperone protein ClpB (ATP-dependent unfoldase) (Variovorax sp. SCN45)
MRQDKLTTKFQEALGDAQSLALGNDNAYIEPAHLLAAMLRQDDGPRALLERAGVNVPGLT
QAAEAAIKKLPQVQGHDIVQVGPELGKLLQATEKEAIKRNDQFIAGELFLLALADSKADI
GKTAKENGLSRKSLEAAIDAVRGGQGVNSADAEGQREALKKYCMDLTERARLGKLDPVIG
RDEEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGEVPDSLKGKRVLSLDM
AALLAGAKFRGEFEERLKTVLNELAKDEGQTIVFIDELHTMVGAGKAEGAMDAGNMLKPA
LARGELHCVGATTLDEYRKYIEKDAALERRFQKIIVGEPSVEATIAILRGLQEKYEVHHG
VQITDPAIVAAAELSDRYITDRFLPDKAIDLIDEAAAKIKIEMDSKPEVMDRLDRRLIQL
QIEREAVRREKDEASQKRLGLIEDEIVKLQKEIADYDEIWQAEKAQAQGSAQVREDVDKI
KFQIEEWKRKGDFNKVAELQYGQLPALEKRLKEAEASEASKGKSSAPTLLRTQVGSEEIA
EVVARATGIPVAKLMQGERDKLLVMEDKLHERVVGQDEAIGAVANAIRRSRSGLSDPNRP
TGSFLFLGPTGVGKTELCKALAGFLFDSEDHLIRIDMSEFMEKHSVARLIGAPPGYVGYE
EGGYLTEAVRRKPYSVVLLDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMT
SNIGSPIIQAMVGKPSEEIKEAVWDELKNYFRPEFLNRIDETVVFHALDAKNIESIAAIQ
LKVLQARLAKMDLGLEVSPAALAEIAKVGFDPVFGARPLKRAIQQRIENPLSKLLLDGSF
GPKDTIEVTTDPIQTPGQFSFTKAGEPTAAASA