Protein Info for GFF2778 in Methylophilus sp. DMC18

Annotation: Tyrosine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 412 TIGR00234: tyrosine--tRNA ligase" amino acids 21 to 411 (391 residues), 396.4 bits, see alignment E=7.9e-123 PF00579: tRNA-synt_1b" amino acids 45 to 328 (284 residues), 281.9 bits, see alignment E=9.6e-88 PF01479: S4" amino acids 354 to 396 (43 residues), 24.8 bits, see alignment 2.2e-09

Best Hits

Swiss-Prot: 68% identical to SYY_NITEU: Tyrosine--tRNA ligase (tyrS) from Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)

KEGG orthology group: K01866, tyrosyl-tRNA synthetase [EC: 6.1.1.1] (inferred from 82% identity to meh:M301_0105)

Predicted SEED Role

"Tyrosyl-tRNA synthetase (EC 6.1.1.1)" (EC 6.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (412 amino acids)

>GFF2778 Tyrosine--tRNA ligase (Methylophilus sp. DMC18)
MTDTKQPSSEVPALSPEIAKALAIIKRGADELLIEQELVEKLKTGKPLRVKAGFDPTAPD
LHLGHTVLINKLRQLQDLGHQILFLIGDFTGMIGDPTGKSATRPPLTVEQVKANADTYAA
QVFKILDPAKTEIVFNSNWLSELGAAGMIKLAASHTVARMLERDDFSKRFKGNQPIAIHE
FLYPLLQGYDSVALQADLELGGTDQKFNLLMGRELQKQAGQSQQCVLMMPLLEGLDGVNK
MSKSLGNYIGISEAPETIFAKIMSISDELMWRYIDLLSFATLETIAEWKAQVASGENPRN
IKVGFAQEIVARFHGQAAAEKALQDFQTRAKGGIPDDVPEVEVTIEGESIGISQLLKQAG
LVESTSEAMRAVQQGGVKLDSVKVDDKNLPLTKGVTVIAQVGKRKFAKITIR