Protein Info for GFF2776 in Xanthobacter sp. DMC5

Annotation: Nitrilase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 56% identical to NITR2_BRADU: Nitrilase blr3397 (blr3397) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01501, nitrilase [EC: 3.5.5.1] (inferred from 62% identity to sfu:Sfum_2312)

MetaCyc: 51% identical to Nit1 (Arabidopsis thaliana col)
Nitrile hydratase. [EC: 4.2.1.84]; Nitrilase. [EC: 4.2.1.84, 3.5.5.1]; 3.5.5.1 [EC: 4.2.1.84, 3.5.5.1]

Predicted SEED Role

"Plant-induced nitrilase (EC 3.5.5.1), hydrolyses beta-cyano-L-alanine" (EC 3.5.5.1)

MetaCyc Pathways

superpathway of acrylonitrile degradation (3/3 steps found)
acrylonitrile degradation I (2/2 steps found)
indole-3-acetate biosynthesis IV (bacteria) (2/2 steps found)
indole-3-acetate biosynthesis V (bacteria and fungi) (1/1 steps found)
aldoxime degradation (2/3 steps found)
indole glucosinolate activation (herbivore attack) (8/13 steps found)
indole-3-acetate biosynthesis II (6/12 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.5.1, 4.2.1.84

Use Curated BLAST to search for 3.5.5.1 or 4.2.1.84

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (314 amino acids)

>GFF2776 Nitrilase (Xanthobacter sp. DMC5)
MSKLKVAVVQAATEGADSEATLKKAERLIAECGSKGVAVAVFPEAFIGGYPKGADFQICV
GMRRPGTREEFLGYASRAIAVPGPATERLGSAVRAAGIYLTIGVIEKDGGTLYCTALFFG
PDGSLLGKHRKTLPTAAERLVWGSGDGSTLAAVDTPWGPMGAVICWESYVPMLRMAMYSK
GIALYCAPTADDRDTWAPSMQHVAMEGRCFVLSACQYMSRGNFPGDIRNTITDNDEDVLM
RGGSLIVGPLGNIIAGPDFSGETILTADIDTDDITRAQFDHDVNGHYSRPDLFKLIVNEE
PLKSVVPVKGMAGH