Protein Info for GFF2775 in Variovorax sp. SCN45

Annotation: Lignostilbene-alpha,beta-dioxygenase and related enzymes

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 PF03055: RPE65" amino acids 26 to 482 (457 residues), 446 bits, see alignment E=1e-137

Best Hits

KEGG orthology group: K11159, carotenoid cleavage dioxygenase (inferred from 45% identity to vpe:Varpa_0018)

Predicted SEED Role

"Lignostilbene-alpha,beta-dioxygenase and related enzymes" in subsystem Phenylpropanoid compound degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (484 amino acids)

>GFF2775 Lignostilbene-alpha,beta-dioxygenase and related enzymes (Variovorax sp. SCN45)
VTAATTTTSAAATQAGNAYFFSGNYAALTQEHDIAALDVVGAIPPELSGTLYRVGPSPQY
APRDDNYHWFSGDGMVHAFHVDGGKVSYSNRWARTPKWQLENEAGRALFGSFGNPATSDP
AALGRNSGTANTSMLWHGGRLFALEESHQPFELDALTLASKGHQSFGERLTARCTAHPKI
DPETGELHFFAYSPEGPGTPGMLYGVMDSACEITELQSFTAPYASMAHDFMVTRGHVLFP
VMPLTTSVERAMRGKPLLAWDADKRTHVGVMRRGADAGSMRWFEADACHVFHVMNAWDDG
DSIVAFVMQSDTAPGLPDSEGRPGDPHAMAARLCRWTFDLASPGTGFRREYLDDLVAEFP
RIDERHAGRRNRYGFYTCHATARARGDAESVLFDSLARFDFDTGERSIHTLPAGDVMSEP
VFVPRSADAAEGDGWLLAVAWREREKRSDLLVLDACDLGAAPVAVARLPHRVPFGFHGNW
RPNA