Protein Info for GFF2755 in Methylophilus sp. DMC18

Annotation: Exopolyphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 492 TIGR03706: exopolyphosphatase" amino acids 10 to 303 (294 residues), 309.1 bits, see alignment E=1.6e-96 PF02541: Ppx-GppA" amino acids 26 to 304 (279 residues), 244.2 bits, see alignment E=1.9e-76 PF21447: Ppx-GppA_III" amino acids 313 to 476 (164 residues), 112.9 bits, see alignment E=1.5e-36

Best Hits

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 55% identity to adn:Alide_2491)

Predicted SEED Role

"Exopolyphosphatase (EC 3.6.1.11)" in subsystem Phosphate metabolism (EC 3.6.1.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (492 amino acids)

>GFF2755 Exopolyphosphatase (Methylophilus sp. DMC18)
MLNINHDTQLAAVDLGSNSFRLEIGRVVDGQILRVDYLKEAVRQGGDLDEDRNLKPQAFE
RGLKCLARFGERLKDFPVEHVRAVATQTLREATNSEDFIRQAQKVLGYPIEIISGVEEAR
LIYQGVSRMLPQSDEKRLVVDIGGRSTEFILGQHFRATVTESLRLGSVGWSQKYFADGQF
SERNFERAEIAAESIIDMIANRYVGKAEVAYGASGTVGAVADVLAGAGFAADRIERAQLE
WLKQTLIKAKSPDRLNMEGLKDDRRAVIGGGLAVLTAVFDTLKIDTLMVANGALRHGVLY
DMLNPEDVTEDLRTASIARLCKQFGVEMEHANNVSKMALYFYDAMLACDTQKPLLHWAAL
CHEIGWAISHTDCNKHGAYILDNTELMGFAQSELHTISLLVLGHQGKLRKLDADFDNQDL
VCQLMALRLAVIFCHSRRMPSLKHLQLERHKQQFILALSTQWANQHPQTHYLLEEECLLW
QKLGWQLEIRHT