Protein Info for GFF2743 in Methylophilus sp. DMC18

Annotation: Pyridoxine 5'-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 242 TIGR00559: pyridoxine 5'-phosphate synthase" amino acids 3 to 238 (236 residues), 309.6 bits, see alignment E=7.3e-97 PF03740: PdxJ" amino acids 3 to 236 (234 residues), 338 bits, see alignment E=1.1e-105

Best Hits

Swiss-Prot: 65% identical to PDXJ_CUPMC: Pyridoxine 5'-phosphate synthase (pdxJ) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K03474, pyridoxine 5-phosphate synthase [EC: 2.6.99.2] (inferred from 88% identity to mmb:Mmol_1630)

MetaCyc: 59% identical to pyridoxine 5'-phosphate synthase (Escherichia coli K-12 substr. MG1655)
Pyridoxine 5'-phosphate synthase. [EC: 2.6.99.2]

Predicted SEED Role

"Pyridoxine 5'-phosphate synthase (EC 2.6.99.2)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 2.6.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (242 amino acids)

>GFF2743 Pyridoxine 5'-phosphate synthase (Methylophilus sp. DMC18)
MLKLGVNIDHVATIRQARGTKYPSVVQAALRAEQSGADSITLHLREDRRHMQDADIFALR
PLLQTKMNLECAVTSEMIDIAIQVKPQDVCLVPERREERTTEGGLDVLTHYAKVEHAVKK
LSEQGIRVSLFIAPDLAQIDAAAKMGAPVIELHTGTFADAETEKAQLAELDKITTALNHA
KAAGLVVNAGHGLHYHNVHHIARLPGFEELNIGHAIVAHALFVGWDHAVREMKALMKEFS
SR