Protein Info for GFF2733 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01565: FAD_binding_4
  • amino acids 57 to 195 (139 residues), 132.3 bits, E=1e-42
• PF02913: FAD-oxidase_C
  • amino acids 236 to 472 (237 residues), 219.6 bits, E=5.5e-69

Best Hits

Swiss-Prot: 61% identical to GLCD_ECOLI: Glycolate oxidase subunit GlcD (glcD) from Escherichia coli (strain K12)

KEGG orthology group: K00104, glycolate oxidase [EC: 1.1.3.15] (inferred from 86% identity to pol:Bpro_0433)

MetaCyc: 61% identical to glycolate dehydrogenase, putative FAD-linked subunit (Escherichia coli K-12 substr. MG1655)
Glycolate dehydrogenase. [EC: 1.1.99.14]; 1.1.99.14 [EC: 1.1.99.14]

Predicted SEED Role

"Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)

MetaCyc Pathways

• glycolate and glyoxylate degradation II (2/2 steps found)
• glycolate and glyoxylate degradation I (3/4 steps found)
• superpathway of glycol metabolism and degradation (5/7 steps found)
• glycolate and glyoxylate degradation III (1/3 steps found)
• photorespiration I (4/9 steps found)
• photorespiration III (4/9 steps found)
• photorespiration II (4/10 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.3.15, 1.1.99.14

Use Curated BLAST to search for 1.1.3.15 or 1.1.99.14

Protein Sequence (500 amino acids)

>GFF2733 Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNAPALPAENTFLQRAQRQADVVRALQAVVPTHALLWQAEDTVPYECDGLTAYRERPLVV
VLPETEDQVAAVLKACHGLGVPVVARGAGTGLSGGAMPHALGVTLSLAKFNRIVRIDPRA
RTAVVQAGVRNLAISEAAAPHGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTLHNV
LSVRGFTVEGEPVTFGGDALDVPGLDLLPLVIGSEGMLAVTTEVTVKLVPKPLLARCIMA
SFDDMRKAGDAVASVIAAGIIPAGLEMMDKPMTAAVEDFVHAGYDLDAEAILLCESDGTP
EEVEEEIGRMSEVLRRCGATAIAVSRDEAERLRFWSGRKNAFPASGRISPDYMCMDSTIP
RKRLADILLAIAGMEKKYGLRCANVFHAGDGNLHPLIMFDANDADQLHRCELFGAEILET
SVAMGGTVTGEHGVGVEKLNSMCVQFSAEENAQMFAVKRAFDTNGLLNPGKVIPTLQRCA
EYGRMLVRAGALAHPELPRF