Protein Info for GFF2730 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Biotin carboxylase (EC 6.3.4.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 609 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF13533: Biotin_lipoyl_2" amino acids 4 to 41 (38 residues), 27.7 bits, see alignment 2.8e-10 PF00364: Biotin_lipoyl" amino acids 5 to 67 (63 residues), 56.3 bits, see alignment E=3.5e-19 PF01039: Carboxyl_trans" amino acids 121 to 592 (472 residues), 254.5 bits, see alignment E=2.9e-79

Best Hits

KEGG orthology group: None (inferred from 81% identity to rfr:Rfer_3899)

Predicted SEED Role

"Biotin carboxylase (EC 6.3.4.14)" (EC 6.3.4.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14

Use Curated BLAST to search for 6.3.4.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (609 amino acids)

>GFF2730 Biotin carboxylase (EC 6.3.4.14) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNSLRSPLAAQIVQWLVAPGDQVRQGDVLVILEAMKMEHELRAETAAQVLELLFAPGESV
QVGDVLLRATPARPSDLPAAASAEAPAAAAGPAALRPDLQRLIDRQARTLDAARPEAVAR
RHALGLRTARENIADLCDPDSFLEYGALAVAAQRNRRSEEDLVANTPADGMVTGIGSVNA
AQHGAERSRTAVMAYDATVLAGTQGMRNHQKTDRLLGIALEQRLPVVLLAEGGGGRPGDT
DMPIVAGLHVGTFAAFARLCGQVPVLGIVSGRCFAGNAALLGCCDVVIATRGSNIGMGGP
AMVEGGGLGVFRPEQIGPSAVQHANGVIDVLVEDEAQAVAAARHYLSFFQGPVDGGRAPD
ALALREVVPENRLRVYDTRAAMDGLVDAGSLLPLRTGFGIGIHTALARIEGRAVGVMANN
PLHLGGAIDADAADKAARFMQLCNAHGLPIVSLVDTPGFMVGPDIEARAQVRHVSRLFVG
AANLRVPFFSVVLRKGYGLGAMGMTAGGFHAPAFTVAWPTAEFGAMGLEGAVRLGYRKEL
EAVPQGPQRDALFERLLAQQYANGSAVQMATTLEIDAVIDPAHTRDWLVKGLAGAKVSAH
RAGRFIDTW