Protein Info for GFF2715 in Variovorax sp. SCN45

Annotation: 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 327 PF00389: 2-Hacid_dh" amino acids 7 to 319 (313 residues), 100 bits, see alignment E=1.8e-32 PF02826: 2-Hacid_dh_C" amino acids 109 to 291 (183 residues), 193.1 bits, see alignment E=5.7e-61 PF03807: F420_oxidored" amino acids 148 to 218 (71 residues), 24.5 bits, see alignment E=7e-09 PF03446: NAD_binding_2" amino acids 148 to 250 (103 residues), 25 bits, see alignment E=3.6e-09

Best Hits

Swiss-Prot: 52% identical to GHRB_CITK8: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K00090, gluconate 2-dehydrogenase [EC: 1.1.1.215] (inferred from 94% identity to vpe:Varpa_2201)

MetaCyc: 57% identical to 2-dehydro-6-phosphogluconate reductase (Pseudomonas fluorescens)
Phosphogluconate 2-dehydrogenase. [EC: 1.1.1.43]

Predicted SEED Role

"2-ketogluconate 6-phosphate reductase (EC 1.1.1.43)" in subsystem 2-Ketogluconate Utilization (EC 1.1.1.43)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.215

Use Curated BLAST to search for 1.1.1.215 or 1.1.1.43

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (327 amino acids)

>GFF2715 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43) (Variovorax sp. SCN45)
MTQQKNILVFRPLPEDQLARLQAAHHVTVADPRKEPEAFAAALRTADGLIGSSHPVDAAL
LDAAPQLQVISSVSVGVDNYPLAELRKRGIVLCHTPDVLTETVADTVFAILMATQRRVVE
LSNLVREGRWTKNIGEELFGTDVHGKTLGILGFGRIGQAVARRAALGFGMPVLYHSRRPV
DLAAQAPELQGRAAHTPLDDLLARADIVLAMLPLTDATRGMIDAAFFARMKPGAAFINGG
RGATVNEEALLNALDHGTLRAAGLDVFAKEPLPADSPLRTHPRVTPLPHIGSATHETRHA
MAELATTNLLQVLAGEKPTAPYDTASA