Protein Info for GFF2710 in Methylophilus sp. DMC18

Annotation: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 PF00919: UPF0004" amino acids 1 to 91 (91 residues), 82.6 bits, see alignment E=2.5e-27 TIGR01574: tRNA-i(6)A37 thiotransferase enzyme MiaB" amino acids 1 to 427 (427 residues), 533 bits, see alignment E=6.2e-164 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 1 to 418 (418 residues), 437.4 bits, see alignment E=5.4e-135 PF04055: Radical_SAM" amino acids 139 to 310 (172 residues), 85.1 bits, see alignment E=9.5e-28 PF01938: TRAM" amino acids 364 to 425 (62 residues), 37.1 bits, see alignment E=3.5e-13

Best Hits

Swiss-Prot: 70% identical to MIAB_METFK: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (miaB) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K06168, bifunctional enzyme involved in thiolation and methylation of tRNA (inferred from 78% identity to mmb:Mmol_1865)

MetaCyc: 58% identical to isopentenyl-adenosine A37 tRNA methylthiolase (Escherichia coli K-12 substr. MG1655)
RXN0-5063 [EC: 2.8.4.3]

Predicted SEED Role

"tRNA-i(6)A37 methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase or tRNA processing

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (428 amino acids)

>GFF2710 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (Methylophilus sp. DMC18)
MNEYDASRMIDLMREHEGMTQTENMEEADVILLNTCSVREKAEEKVFSHLGRMIPLKEKN
PNLVIGVGGCVASQEGEAIIQRAPYVDVVFGPQTLHRLPELIKAKQAADKPQVDIAFPEI
EKFDHLPPPRVEGATAFLSIMEGCSKYCSFCVVPYTRGEEFSRPFEDIITEAIQLAEQGV
KEITLLGQNVNAYRVPYQGIEADLPLLIETIAEIPQIERIRYTTSHPNEMSERLIACHAT
VPKLANQLHLPVQAGSDRILMAMKRNYTALQYKGLIRKLKAANPALTITSDIIVGFPGET
EKDFQATLKLMRDVGFDYSFSFLYSPRPGTPASYLPDETPQADKAAWLKQLQTENETNGD
AISRAMLGTVQRVLIDGPSWKDPNLLAGKTDNGRVVDIAADARYIHQMVQVKITDVYNPK
RLVGEIVN