Protein Info for GFF2701 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Pyruvate dehydrogenase E1 component (EC 1.2.4.1)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 55% identical to ODP1_ECOLI: Pyruvate dehydrogenase E1 component (aceE) from Escherichia coli (strain K12)
KEGG orthology group: K00163, pyruvate dehydrogenase E1 component [EC: 1.2.4.1] (inferred from 83% identity to dac:Daci_2498)MetaCyc: 55% identical to pyruvate dehydrogenase E1 component (Escherichia coli K-12 substr. MG1655)
Pyruvate dehydrogenase (acetyl-transferring). [EC: 1.2.1.104, 1.2.4.1]; 1.2.1.104 [EC: 1.2.1.104, 1.2.4.1]
Predicted SEED Role
"Pyruvate dehydrogenase E1 component (EC 1.2.4.1)" in subsystem Methionine Degradation or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.4.1)
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
- pyruvate fermentation to acetate II (3/3 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
- photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
- pyruvate fermentation to acetate and (S)-lactate I (3/4 steps found)
- pyruvate fermentation to acetate V (2/3 steps found)
- anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 1.2.4.1
Use Curated BLAST to search for 1.2.1.104 or 1.2.4.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (861 amino acids)
>GFF2701 Pyruvate dehydrogenase E1 component (EC 1.2.4.1) (Hydrogenophaga sp. GW460-11-11-14-LB1) MTDVSTQQHFGDPDPAETLEWREALLSLTASQGPARARQILDELARLARQQRIGWQPELN TPYVNTIAVEDQPAFPGDLAVEERLASLMRWNALAMVVRANQAYGELGGHIASYASAADL FETGFNHFFHARSEQHGGDLVFFQPHSAPGVYARAFLEGRLSEADLRHYRQEITAPADGA RGLSSYPHPWLMPDFWQFPTGSMGIGPISSIYHARFMRYLSHRNLQDCTGRKVWGVFGDG EMDEPESMSALTLAARERLDNLVWVVNCNLQRLDGPVRGNGRIIDELEKLFAGAGWNVIK LVWGSDWDGLFARDVTGALVRAFAHTVDGQMQTFAAKDGRFNRDNFFGQNEELARLAQGM TDEQIDRLKRGGHDLVKIHAAYAAAAAHTGQPTVILAQTKKGYGMGSAGQGKMTTHSQKK FDGADLIEFRNRFNLPLSDEQATGLAFFKPADDSAEMRYLREHRDRLGGAMPRRETVCER VPVPAIGAYASFALKADGKEMSTTMAFVRMLGSLLKDAQLGPRIVPIVADEARTFGMANL FKQVGIYSSVGQRYAPEDIGSVLSYREALDGQILEEGISEAGAIASWTAAATSYSVHGLA MLPFYIYYSMFGFQRVGDAIWAAADQRARGFLLGATSGRTTLGGEGLQHQDGSSHLVAAT IPNCKAYDPAFAGELAVIVDAGMREMLVEQQDVFYYVTLMNENYAQPDLPEGVAGDVLRG GYRFGRFGPEGAAQQVSLLGSGAIFTEVLKAAEQLAAQGVGVEVFSITSWSELARDGVAQ GRDSHLTRLLAGGEGPVIAATDYVRSVPESVRAFMPEGRGYRTLGTDGFGRSDTRAALRA FFQVDARSIANAARAALAGPG