Protein Info for PS417_13430 in Pseudomonas simiae WCS417

Annotation: spermidine/putrescine ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 359 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF01547: SBP_bac_1" amino acids 30 to 294 (265 residues), 54.9 bits, see alignment E=2.1e-18 PF13416: SBP_bac_8" amino acids 36 to 323 (288 residues), 108 bits, see alignment E=1.1e-34 PF13343: SBP_bac_6" amino acids 70 to 300 (231 residues), 54.2 bits, see alignment E=2.2e-18

Best Hits

Swiss-Prot: 60% identical to SPUD_PSEAB: Putrescine-binding periplasmic protein SpuD (spuD) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K11073, putrescine transport system substrate-binding protein (inferred from 96% identity to pfs:PFLU3150)

MetaCyc: 56% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine ABC transporter, periplasmic putrescine-binding protein"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TXJ3 at UniProt or InterPro

Protein Sequence (359 amino acids)

>PS417_13430 spermidine/putrescine ABC transporter substrate-binding protein (Pseudomonas simiae WCS417)
MKAIALLPLMLVASIGQAAETVKIYNWSDYIAPDTTKNFQKETGIGFTYDVYDSNETLDG
KLMTGKSGYDVVFPSNHFMARQIQGGALKKLDKSQLPNWKNLNPVLLKALENNDPGNAHG
FPYLWGSTGIGYNIDKVKAVLGDNAPVDSWDLIFKPENMAKLQKCGVAILDNGPELLPAA
LNYLGLPHHSKKPEDYKKAEDLLMKVRPYVAYFHSSKYTADLANGDICVAVGFSGDILQA
ESRAKEAKNGVNIGYNIPKEGAAIWFDMVAMPADAPNEAAGYAFMNYLLRPQVMASITNY
VHYANGNQAADSLVDPAIKADTKIYPSPEMMGKLFALEAMPLNIDRVRTRVWNTIRTGK