Protein Info for GFF2630 in Sphingobium sp. HT1-2

Annotation: 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00170: 3-isopropylmalate dehydratase, large subunit
  • amino acids 4 to 473 (470 residues), 750.6 bits, E=2.8e-230
• PF00330: Aconitase
  • amino acids 9 to 466 (458 residues), 589 bits, E=3.9e-181

Best Hits

Swiss-Prot: 80% identical to LEUC_NOVAD: 3-isopropylmalate dehydratase large subunit (leuC) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 94% identity to sjp:SJA_C1-28290)

MetaCyc: 61% identical to isopropylmalate isomerase large subunit (Leptospira interrogans serovar Lai str. 56601)
(R)-2-methylmalate dehydratase. [EC: 4.2.1.35]; 3-isopropylmalate dehydratase. [EC: 4.2.1.35, 4.2.1.33]; 4.2.1.33 [EC: 4.2.1.35, 4.2.1.33]; RXN-7744 [EC: 4.2.1.35, 4.2.1.33]

Predicted SEED Role

"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35

Protein Sequence (483 amino acids)

>GFF2630 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) (Sphingobium sp. HT1-2)
MVKPRTLYEKIWDAHVVERRPDGTCLIYIDRHLVHEVTSPQAFEGLRLAGRKVRRPDLTL
AVPDHNLPTTPRRDADGHRIPIADPESAQQLAALERNAPEFGVRLIGDADPEQGIVHVVG
PEQGFTLPGTTLVCGDSHTSSHGALGALAFGIGTSEVEHVLATQTLLLKQSKTMQVVVEG
ELGYGVTPKDVALAICGTIGTAGGTGYVMEYRGSVFRDMSIEGRLTVANMSIEAGARSGL
FAPDEKTFAYIEGRPMAPKGADWDAAVAWWKTLHTDEGAVFDKTVVIDAADIVPNVTWGT
SPEDVVPVTGVVPDPESFADASKRAAAQKSLDYMGLSAGQKMEDVAIEHVFIGSCTNSRI
EDLRAAAGLLKGRHIAAGIKHAMVVPGSGLVKRMAEAEGLDRIFLDAGFEWREPGCSACL
GMNPDKVPAGERCASTSNRNFMGRQGPGSRTHLVSPQMAAAAALTGRLTDVRALMGEKEG
MVA