Protein Info for GFF2617 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02818: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase
  • amino acids 2 to 369 (368 residues), 733.3 bits, E=2.5e-225
• PF08240: ADH_N
  • amino acids 28 to 113 (86 residues), 86.5 bits, E=1.6e-28
• PF00107: ADH_zinc_N
  • amino acids 197 to 326 (130 residues), 83.5 bits, E=2e-27
• PF13602: ADH_zinc_N_2
  • amino acids 231 to 362 (132 residues), 30.3 bits, E=1.2e-10

Best Hits

Swiss-Prot: 81% identical to FRMA_ECOK1: S-(hydroxymethyl)glutathione dehydrogenase (frmA) from Escherichia coli O1:K1 / APEC

KEGG orthology group: K00121, S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase [EC: 1.1.1.1 1.1.1.284] (inferred from 100% identity to seh:SeHA_C1805)

MetaCyc: 81% identical to S-(hydroxymethyl)glutathione dehydrogenase (Escherichia coli K-12 substr. MG1655)
S-(hydroxymethyl)glutathione dehydrogenase. [EC: 1.1.1.284]

Predicted SEED Role

"S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 1.1.1.284)

MetaCyc Pathways

• superpathway of N-acetylneuraminate degradation (22/22 steps found)
• mixed acid fermentation (16/16 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (18/19 steps found)
• superpathway of anaerobic sucrose degradation (17/19 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (9/9 steps found)
• heterolactic fermentation (15/18 steps found)
• (S)-propane-1,2-diol degradation (5/5 steps found)
• ethanolamine utilization (5/5 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• ethanol degradation II (3/3 steps found)
• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• pyruvate fermentation to ethanol I (3/3 steps found)
• pyruvate fermentation to ethanol III (3/3 steps found)
• ethanol degradation I (2/2 steps found)
• pyruvate fermentation to ethanol II (2/2 steps found)
• acetylene degradation (anaerobic) (4/5 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• acetaldehyde biosynthesis I (1/1 steps found)
• phytol degradation (3/4 steps found)
• L-isoleucine degradation II (2/3 steps found)
• L-leucine degradation III (2/3 steps found)
• L-methionine degradation III (2/3 steps found)
• L-valine degradation II (2/3 steps found)
• protein S-nitrosylation and denitrosylation (3/5 steps found)
• L-phenylalanine degradation III (2/4 steps found)
• L-tyrosine degradation III (2/4 steps found)
• salidroside biosynthesis (1/4 steps found)
• serotonin degradation (3/7 steps found)
• phenylethanol biosynthesis (1/5 steps found)
• butanol and isobutanol biosynthesis (engineered) (3/8 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• noradrenaline and adrenaline degradation (4/13 steps found)
• L-tryptophan degradation V (side chain pathway) (1/13 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Drug metabolism - cytochrome P450
• Fatty acid metabolism
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Metabolism of xenobiotics by cytochrome P450
• Methane metabolism
• Retinol metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.1

Use Curated BLAST to search for 1.1.1.1 or 1.1.1.284

Protein Sequence (372 amino acids)

>GFF2617 S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MKSRAAVAFGPGQPLKIVEIDVAPPKKGEVLVKITHTGVCHTDAFTLSGDDPEGVFPAVL
GHEGGGVVVEVGEGVTSLKPGDHVIPLYTAECGECKFCKSGKTNLCQAVRATQGKGLMPD
GTTRFSYNGEPVYHYMGTSTFSEYTVCAEISLAKVNPQAPLDKVCLLGCGVTTGIGAVHN
TAKVKAGDTVAVFGLGGIGLAVIQGAVQAKAGRILAVDTNPEKFTLAGEMGATDFINPND
YDKPIQDVIVELTDGGVDFSFECIGNVNVMRAALECCHKGWGESIIIGVAGAGQEIKTRP
FQLVTGRVWRGSAFGGVKGRTQLPGMVEDAMNGKIRLDPFITHRLPLEQINDAFELMHQG
KSIRTVIHFGDN