Protein Info for GFF2615 in Methylophilus sp. DMC18

Annotation: Proline--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 564 (564 residues), 769.1 bits, E=1.7e-235
• PF00587: tRNA-synt_2b
  • amino acids 95 to 455 (361 residues), 118 bits, E=7.6e-38
• PF04073: tRNA_edit
  • amino acids 257 to 370 (114 residues), 91.2 bits, E=8.3e-30
• PF03129: HGTP_anticodon
  • amino acids 473 to 565 (93 residues), 68.8 bits, E=5.7e-23

Best Hits

Swiss-Prot: 77% identical to SYP_METFK: Proline--tRNA ligase (proS) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 82% identity to mmb:Mmol_0455)

MetaCyc: 56% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• anguibactin biosynthesis (1/8 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• pyochelin biosynthesis (1/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (1/25 steps found)
• phosphinothricin tripeptide biosynthesis (1/25 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Protein Sequence (569 amino acids)

>GFF2615 Proline--tRNA ligase (Methylophilus sp. DMC18)
MRASQFFIATQKEAPSDAELISHKLMVRAGLIKRLGSGLYSWMPLGLRVLRKVENIVRDE
MNKAGALELLMPAVQPKELWEETGRWAVFGPQMLKIQDRHERDFCFGPTHEEVITDIARK
EISSYKQLPLNFYQIQTKFRDEIRPRFGVMRAREFLMKDAYSFHTSVDCLVNTYNTMHTA
YSNVFTRLGLKFRAVKADSGAIGGDGSQEFHVLADSGEDALAYCEQSDYAANVELAEAVA
TGSRAAATEAMREVDTPKQTTCEDVAKLLEVPVSKTVKSVALLVDGVFTLALLRGDHQLN
EVKFGKVEGVKEFRLATEEEIRANLNCPPGFIGPVGVNANVRVVADRTVALMSDFVCGAN
KPKFHLAGVNFGRDLPEPAIVADIRNVVEGDASPDGKGTLSLCRGIEVGHIFQLRTKYSQ
AMQATYLDENGQSQVMEMGCYGIGVSRIVGAAIEQGNDDKGIIFPLSMAPFAVVICPIGL
DKSEAVKQAAFELYAQLQQAGVDVLLDDRGERPGVMFAESDLMGIPHRVVIGDRGLAEGK
VEYKGRTDAEAQNLQVANCLAFLQRKLAE