Protein Info for GFF2598 in Variovorax sp. SCN45
Annotation: 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 46% identical to PCAB_PSEPU: 3-carboxy-cis,cis-muconate cycloisomerase (Fragment) (pcaB) from Pseudomonas putida
KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 96% identity to vpe:Varpa_2295)MetaCyc: 46% identical to subunit of 3-carboxymuconate cycloisomerase (Pseudomonas putida)
3-carboxy-cis,cis-muconate cycloisomerase. [EC: 5.5.1.2]
Predicted SEED Role
"Adenylosuccinate lyase (EC 4.3.2.2)" in subsystem De Novo Purine Biosynthesis or Purine conversions (EC 4.3.2.2)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
- superpathway of purine nucleotide salvage (12/14 steps found)
- inosine-5'-phosphate biosynthesis I (6/6 steps found)
- aromatic compounds degradation via β-ketoadipate (8/9 steps found)
- inosine-5'-phosphate biosynthesis II (5/5 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
- protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
- adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
- inosine-5'-phosphate biosynthesis III (5/6 steps found)
- dZTP biosynthesis (3/5 steps found)
- toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
- superpathway of aerobic toluene degradation (12/30 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Benzoate degradation via hydroxylation
- Biosynthesis of alkaloids derived from histidine and purine
- Purine metabolism
Isozymes
Compare fitness of predicted isozymes for: 4.3.2.2, 5.5.1.2
Use Curated BLAST to search for 4.3.2.2 or 5.5.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (404 amino acids)
>GFF2598 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2) (Variovorax sp. SCN45) MSIFEGFLSTSETLGAFSDRAFVDAMLRFEAALARAQAAEGLIPESAAHSIVSSCKVELF DVAKIVRESGRAGSVAIPLVKALREAVSLFNADAAPYVHFGSTSQDVIDSAMALVTREAV ALIETDLAKAADALLRLATQHAETPMLARTLMQPASVTSFGFKCAGWAAPLVRSRLRLRE AAKHALQLQLGGAVGTLAQMKGQGAAVRQRMAKELGLGDPGATWHTQRDEWVALGCELGL VTGSLGKIAVDIALLGQYEVAEVSEPSEPGRGGSSAMPHKRNPVASMVAIAAAHRAPQRV AALLGAMPQQHERALGAWQAELAEWPQLLMSAHGSVRAMAGALPGLQVDAARMRANIDRL RAELPRDAADEWFDPALAHNAGQTALAEVKALQARLDADKELSQ