Protein Info for GFF2588 in Variovorax sp. SCN45

Annotation: Methionyl-tRNA synthetase (EC 6.1.1.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR00398: methionine--tRNA ligase" amino acids 8 to 547 (540 residues), 527.6 bits, see alignment E=3.6e-162 PF09334: tRNA-synt_1g" amino acids 8 to 410 (403 residues), 468.6 bits, see alignment E=2.8e-144 PF00133: tRNA-synt_1" amino acids 10 to 65 (56 residues), 35.8 bits, see alignment 8.6e-13 PF19303: Anticodon_3" amino acids 423 to 549 (127 residues), 28 bits, see alignment E=3.7e-10 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 567 to 698 (132 residues), 134.1 bits, see alignment E=3e-43 PF01588: tRNA_bind" amino acids 598 to 678 (81 residues), 82.1 bits, see alignment E=5e-27

Best Hits

Swiss-Prot: 75% identical to SYM_ACIAC: Methionine--tRNA ligase (metG) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 95% identity to vpe:Varpa_4753)

MetaCyc: 52% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (698 amino acids)

>GFF2588 Methionyl-tRNA synthetase (EC 6.1.1.10) (Variovorax sp. SCN45)
MSAPRKLFVTTALPYANGKFHIGHMMEYIQADIWVRNQRMNGADVNFVCADDAHGAAITI
AADKAGVTPQAFVAEIAAGRKPYLDGYHIAFDNWHSTDAPENHQLAQDIYRDLRANGLIA
TKSVEQFYDPQKGMFLADRFIKGECPNCHSKDQYGDNCEVCGAVYAPTELINPYSALSGA
KPELRSSEHFFFKLSDPRCEAYLKEWTAAPGHVQPEVQNKIREWLYKDDEGKGGLGDWDI
SRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLDKRCIELGGDGISYTEYMADPEL
EQVHFIGKDIITFHTLFWPAMLHFSGRKAPNAVYVHGHLTVSGEKMSKSRGTGIDPLKYL
SIGLDPEWLRYYIAAKLNGRNEDIDFNPEDFVARVNSDLVGKYINIASRAAGFIGKRFGG
KLGEVSADGDALLFTLRDAAQQIHSLYDGRDYARALREVMALADKVNEYVDQNKPWELAK
KEGAEARLHDVCTVCIEAFRLLTLYLKPVLPALALNVEAFLKTSPLVWADATQSLPAGHA
IGDYKHLMQRADPKQVDKLFDAPEAAAAPAVATQAQELPGGEAIAPTITIDDFAKIDLRI
AKIVACEKVEGSTKLLRLTLDAGEGKTRNVFSGIASAYQPEQLVGKLTVLVANLAPRKMK
FGVSEGMVLAASHGDEKAHPGIHVLEPWPGATPGMRVR