Protein Info for PS417_13170 in Pseudomonas simiae WCS417

Annotation: multidrug DMT transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 transmembrane" amino acids 15 to 36 (22 residues), see Phobius details PF13473: Cupredoxin_1" amino acids 32 to 133 (102 residues), 63.5 bits, see alignment E=1.7e-21 PF09375: Peptidase_M75" amino acids 168 to 387 (220 residues), 88.6 bits, see alignment E=5.5e-29

Best Hits

KEGG orthology group: None (inferred from 93% identity to pfs:PFLU2920)

Predicted SEED Role

"Ferrous iron transport periplasmic protein EfeO, contains peptidase-M75 domain and (frequently) cupredoxin-like domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UF69 at UniProt or InterPro

Protein Sequence (400 amino acids)

>PS417_13170 multidrug DMT transporter permease (Pseudomonas simiae WCS417)
MSNPTPQPSSPPRALRWAVAGSVVVMIAAGGLFYYASQLAASKRQTNHNEIAVTLHGHAC
EPNELSVPAGRASFRIINRSDRAVEWEILDGVLVVEERENIAPGLSQVINANLLPGDYAI
TCGLLSNPRGTLHVTPTAESDAQAKAKPSMVAFIGPLSEFRVYLSGQGSALIKAVTALQQ
AIDAGDLAQAQALYVPAREAYQRLAPASQRLAELDNAINARADYFEKREQDPAFSGFHRL
EYSLFQQRSLDGLAPIAQRLLTDVTTLKQQLLAQSLPPEQLVSIVVRNLNSLADVRAVSG
EEERYSHVDLSGFAANLEAAHKVVDLMRPLLTKSAADLLPNVDSAFTAFDSQLNELKVDG
QYRHYDSVTADQRQQIADKAKALAAALDGIDPALGLSGLK