Protein Info for GFF2577 in Variovorax sp. SCN45

Annotation: 7-carboxy-7-deazaguanine synthase (EC 4.3.99.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 212 TIGR04508: 7-carboxy-7-deazaguanine synthase" amino acids 3 to 212 (210 residues), 334.4 bits, see alignment E=1.5e-104

Best Hits

Swiss-Prot: 62% identical to QUEE_BORPE: 7-carboxy-7-deazaguanine synthase (queE) from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

KEGG orthology group: None (inferred from 94% identity to vap:Vapar_4095)

Predicted SEED Role

"Queuosine Biosynthesis QueE Radical SAM" in subsystem Queuosine-Archaeosine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (212 amino acids)

>GFF2577 7-carboxy-7-deazaguanine synthase (EC 4.3.99.3) (Variovorax sp. SCN45)
MSYSVKEIFYTLQGEGGQAGMPAVFCRFAGCNLWSGRESDRDTAVCRFCDTDFVGTDGTL
GGKFKTADLLADTIAAQWPAGDADHRLVVLTGGEPLLQVDAALVDALHARRFRIAVESNG
TVEAPEGIDWLCISPKAGAPWVQQRGQELKLVWPQTEFDLDTMARTGEFTHRFLQPMDGP
DRIANTELCIAECMRQPAWRLSVQTHKITGIR