Protein Info for GFF256 in Variovorax sp. SCN45

Annotation: Twitching motility protein PilT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 TIGR01420: twitching motility protein" amino acids 4 to 343 (340 residues), 383 bits, see alignment E=7e-119 PF00437: T2SSE" amino acids 5 to 272 (268 residues), 130.3 bits, see alignment E=3.7e-42

Best Hits

KEGG orthology group: K02670, twitching motility protein PilU (inferred from 88% identity to vap:Vapar_2718)

Predicted SEED Role

"Twitching motility protein PilT" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (382 amino acids)

>GFF256 Twitching motility protein PilT (Variovorax sp. SCN45)
MNMMERILRLMAERRASDIYLSAHSPVLIRINGNCVPVNAQVLPPSAPLALLAEVAPAER
IQELESTGELNMALALEGTGNYRISAMRQRGSYAVVVRYISHTIPRFADLNLPDILKTLI
MERRGLILMVGATGAGKTTTLASMLDYRNENASGHIITVEEPIEFTYTNKKSVVNQRDVG
SDTASLQTALKNALRQAPDVIQIGEIRDRDTMTAAIAYAQSGHLCVATLHANNSYRALNR
ILGFYPVEVRATLLGDLGGALRAVVSQRLLRTQAGSRVPAVEVMLNTALVAELIEKGDFS
GVKEAMEKSMAEGSQTFEEDIARLITEERVTREEGLAQADSPTNLLWRLQNRAAPKAAAD
DRSLTDQVDEPTFTDITLDVRF