Protein Info for GFF2555 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: GDP-L-fucose synthetase (EC 1.1.1.271)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 65% identical to FCL_ECOLI: GDP-L-fucose synthase (fcl) from Escherichia coli (strain K12)
KEGG orthology group: K02377, GDP-L-fucose synthase [EC: 1.1.1.271] (inferred from 75% identity to pna:Pnap_3191)MetaCyc: 65% identical to GDP-L-fucose synthase (Escherichia coli K-12 substr. MG1655)
GDP-L-fucose synthase. [EC: 1.1.1.271]
Predicted SEED Role
"GDP-L-fucose synthetase (EC 1.1.1.271)" in subsystem Capsular heptose biosynthesis or Colanic acid biosynthesis (EC 1.1.1.271)
MetaCyc Pathways
- colanic acid building blocks biosynthesis (10/11 steps found)
- GDP-L-fucose biosynthesis I (from GDP-D-mannose) (3/3 steps found)
- superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (8/14 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.271
Use Curated BLAST to search for 1.1.1.271
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (325 amino acids)
>GFF2555 GDP-L-fucose synthetase (EC 1.1.1.271) (Hydrogenophaga sp. GW460-11-11-14-LB1) MNTPETIYVAGHRGMVGSAIVRQLRAAGHPASHIVTRTHTELNLTDQAAVRAFFARTKPQ QVYLAAAKVGGIHANSVFPADFIYQNLMIAANVIEAAFCNGVRKLLFLGSSCIYPKLAEQ PMSEQALLTAPLEATNEPYAVAKIAGIKLCESYNRQFGASHGVDYRSVMPTNLYGPGDNY HPENSHVIPALIRRFHQARQSGVPKVAIWGSGKPMREFLYVDDMATACVHVMNLPKARYE QHTQPMLSHINVGSGSDMTIAELAHRVAHVTGFDGDISFDASKPDGTPRKLMDSSRLHSL GWQAKVDLPAGLALTYQDFLQQAVL