Protein Info for GFF2531 in Pseudomonas sp. DMC3

Annotation: Chaperone protein HtpG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 634 PF02518: HATPase_c" amino acids 32 to 184 (153 residues), 29.8 bits, see alignment E=1.1e-10 PF13589: HATPase_c_3" amino acids 33 to 158 (126 residues), 38.1 bits, see alignment E=2.4e-13 PF00183: HSP90" amino acids 227 to 629 (403 residues), 430.8 bits, see alignment E=1.4e-132

Best Hits

Swiss-Prot: 98% identical to HTPG_PSEPF: Chaperone protein HtpG (htpG) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K04079, molecular chaperone HtpG (inferred from 98% identity to pfo:Pfl01_1625)

MetaCyc: 60% identical to chaperone protein HtpG (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein HtpG" in subsystem Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (634 amino acids)

>GFF2531 Chaperone protein HtpG (Pseudomonas sp. DMC3)
MSVETQKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISNASDAVDKLRFEALAKPELL
EGGADLKIRVSFDKDAKTVTLEDNGIGMNRDDVITHLGTIAKSGTADFMKNLSGDQKKDS
HLIGQFGVGFYSAFIVADKVDVYSRRAGTAASEGVHWSSKGEGEFEVATIDKPERGTRIV
LHLKSGEDEFADGWRLRNIIKKYSDHIALPIELPKEVTAAEGEEAPAVEWETVNRASALW
TRPRTEVKDEEYQEFYKHIAHDFENPLSWSHNKVEGKLEYSSLLYVPARAPFDLYQREAP
KGLKLYVQRVFVMDQAESFLPLYLRFIKGVVDSNDLSLNVSREILQKDPIIDSMKTALTK
RVLDMLEKLAKNEPEQYKGFWKNFGQVMKEGPAEDFANKEKIAGLLRFASTQGDDGEQVV
GLADYLARAKEGQDKIYYLTGETYAQVKNSPHLEVFRKKGIEVLLLTDRIDEWLMSYLSE
FDGKTFVDVARGDLDLGNLDSEEDKKAAEEVAKSKEGLVERLKTALGDSVAEVRVSHRLT
DSPAILAIGEQDLGLQMRQILEASGQKVPDSKPIFEFNPSHPLIEKLDGEQSEERFGDLS
HILFDQAALAAGDSLKDPAAYVRRLNKLLVELSV