Protein Info for GFF2523 in Xanthobacter sp. DMC5

Annotation: Glutamate synthase [NADPH] small chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01317: glutamate synthase, NADH/NADPH, small subunit
  • amino acids 3 to 472 (470 residues), 637.4 bits, E=8.2e-196
• PF14691: Fer4_20
  • amino acids 24 to 133 (110 residues), 87 bits, E=5.3e-28
• PF07992: Pyr_redox_2
  • amino acids 146 to 454 (309 residues), 97.3 bits, E=7.7e-31
• PF01266: DAO
  • amino acids 147 to 181 (35 residues), 28.5 bits, 7.5e-10
• PF01494: FAD_binding_3
  • amino acids 147 to 179 (33 residues), 26.1 bits, see alignment (E = 3.2e-09)
• PF00070: Pyr_redox
  • amino acids 147 to 218 (72 residues), 25.3 bits, E=1.1e-08
• PF12831: FAD_oxidored
  • amino acids 148 to 189 (42 residues), 29 bits, 4.5e-10
• PF00890: FAD_binding_2
  • amino acids 148 to 182 (35 residues), 27.2 bits, 1.5e-09
• PF13450: NAD_binding_8
  • amino acids 150 to 185 (36 residues), 38.3 bits, 8.4e-13
• PF01593: Amino_oxidase
  • amino acids 156 to 185 (30 residues), 30.1 bits, see alignment (E = 2.2e-10)

Best Hits

Swiss-Prot: 50% identical to GLTD_MYCTU: Glutamate synthase [NADPH] small chain (gltD) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 94% identity to xau:Xaut_0476)

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14

Protein Sequence (473 amino acids)

>GFF2523 Glutamate synthase [NADPH] small chain (Xanthobacter sp. DMC5)
MGKVTGFLEIDRREQKYQPASDRIRHFREFTLPLPDAEVENQASRCMDCGIPFCHGSTGC
PVNNQIPDWNDLVYRNEWEEASRNLHSTNNFPEFTGRICPAPCEEACTLNLEDVPVTIKT
VEQAIADKAWKKGWIKPEPAAVKTGKKVAIIGSGPAGLAAAQQLARAGHDVHVYEREPKA
GGLLRYGIPDFKMEKHHIDRRVKQMEAEGVTFHYNENIGVVRPLQELLDGHDAVLLAGGS
EAPRDPGLPGQEMEGVHFAMPYLVQSNRREGDEPVNEIPILAGGKHVVVVGGGDTASDCV
GTAFRQGALSVTQLDIRAVPPMKEDKLAVWPFWPTKFRTSSSQAEGAEREFACATLGIEG
RNGRVTGVKCARVDAKRQPIPGTEFVLRADLVFLAIGFAHPLFSGLIEQSGAALDKRGNV
KADEEAYATSVPKLYAAGDMRRGQSLVVWAIREGRQAAHAIDKDLMGATTLPR