Protein Info for GFF2523 in Variovorax sp. SCN45

Annotation: Peptide chain release factor N(5)-glutamine methyltransferase (EC 2.1.1.297)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 283 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR00536: methyltransferase, HemK family" amino acids 12 to 281 (270 residues), 241.3 bits, see alignment E=1.1e-75 PF17827: PrmC_N" amino acids 14 to 80 (67 residues), 50.7 bits, see alignment E=6.3e-17 TIGR03534: protein-(glutamine-N5) methyltransferase, release factor-specific" amino acids 27 to 274 (248 residues), 280.3 bits, see alignment E=1.4e-87 PF05175: MTS" amino acids 110 to 196 (87 residues), 53.7 bits, see alignment E=6.3e-18 PF06325: PrmA" amino acids 117 to 189 (73 residues), 26.5 bits, see alignment E=1.3e-09 PF13847: Methyltransf_31" amino acids 119 to 186 (68 residues), 36.3 bits, see alignment E=1.4e-12 PF13649: Methyltransf_25" amino acids 121 to 178 (58 residues), 36.5 bits, see alignment E=1.9e-12 PF08241: Methyltransf_11" amino acids 122 to 174 (53 residues), 23.6 bits, see alignment E=2e-08

Best Hits

Swiss-Prot: 53% identical to PRMC_BORPE: Release factor glutamine methyltransferase (prmC) from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

KEGG orthology group: K02493, methyltransferase [EC: 2.1.1.-] (inferred from 86% identity to vpe:Varpa_4819)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (283 amino acids)

>GFF2523 Peptide chain release factor N(5)-glutamine methyltransferase (EC 2.1.1.297) (Variovorax sp. SCN45)
MTVDNPKPSTVAQALAAAIALGIDRLDAQLLLLHALGRAPHDRAWLLAHDTDALRDEAWS
ALSTQLSRRLAGEPVAYLLGEKEFHGLDLRVDARVLVPRPDTETLVEWALECLEGHAAPR
VLDLGTGSGAIALALQHARADAQVDAVDTSADALAVAQANAQRLGLPVRFALANWLDGAE
TGYAVIASNPPYIAANDPHLPALRHEPVSALVAGTDGLDDIRQIVRHAPAHLAEGGWLLL
EHGHDQAAAVRQLLAERGFAEVQSREDLAGIQRCSGGIWRTVK