Protein Info for GFF2522 in Pseudomonas sp. DMC3

Annotation: Dihydrolipoyl dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01350: dihydrolipoyl dehydrogenase
  • amino acids 4 to 472 (469 residues), 532 bits, E=5.8e-164
• PF07992: Pyr_redox_2
  • amino acids 5 to 334 (330 residues), 240.5 bits, E=1.3e-74
• PF12831: FAD_oxidored
  • amino acids 6 to 37 (32 residues), 28.2 bits, see alignment (E = 5.4e-10)
• PF00890: FAD_binding_2
  • amino acids 6 to 44 (39 residues), 28.6 bits, 3.9e-10
• PF01134: GIDA
  • amino acids 6 to 61 (56 residues), 26.5 bits, 1.5e-09
• PF13738: Pyr_redox_3
  • amino acids 139 to 318 (180 residues), 33.1 bits, E=1.6e-11
• PF00070: Pyr_redox
  • amino acids 183 to 257 (75 residues), 62.1 bits, E=2.6e-20
• PF02852: Pyr_redox_dim
  • amino acids 353 to 462 (110 residues), 137.7 bits, E=8.1e-44

Best Hits

Swiss-Prot: 94% identical to DLDH2_PSEPU: Dihydrolipoyl dehydrogenase (lpdG) from Pseudomonas putida

KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 98% identity to pfo:Pfl01_1616)

MetaCyc: 48% identical to dihydrolipoyl dehydrogenase subunit (Syntrophotalea carbinolica DSM 2380)

Predicted SEED Role

"Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4)" in subsystem TCA Cycle (EC 1.8.1.4)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (3/3 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.4

Use Curated BLAST to search for 1.8.1.4

Protein Sequence (478 amino acids)

>GFF2522 Dihydrolipoyl dehydrogenase (Pseudomonas sp. DMC3)
MSQKFDVVVIGAGPGGYVAAIKAAQLGLTTACIEKYTDAEGKQALGGTCLNVGCIPSKAL
LDSSWKYKEAKESFNVHGISTGEVKMDVAAMVGRKAGIVKNLTGGVATLFKANGVTSIQG
HGKLLAGKKVEVTKPDGSVEVIEAENVILAPGSRPIDIPPAPVDQKVIVDSTGALEFQSV
PKRLGVIGAGVIGLELGSVWSRLGAEVTVLEALDTFLMAADTAVSKEALKTLTKQGLDIK
LGARVTGSKVNGEEVVVNYTDANGEQTITFDKLIVAVGRRPVTTDLLSADCGVEIDERGF
IAVDDHCVTAVPGVFAIGDVVRGMMLAHKASEEGIMVVERIKGHKAQMNYDLIPSVIYTH
PEIAWVGKTEQALKAEGVEVNVGTFPFAASGRAMAANDTGGFVKVIADAKTDRVLGVHVI
GPSAAELVQQGAIGMEFGTSAEDLGMMVFSHPTLSEALHEAALAVNGGAIHIANRKKR