Protein Info for PS417_12795 in Pseudomonas simiae WCS417

Annotation: alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 660 PF11896: GlgE_dom_N_S" amino acids 18 to 200 (183 residues), 171.9 bits, see alignment E=2.7e-54 PF00128: Alpha-amylase" amino acids 212 to 336 (125 residues), 30.9 bits, see alignment E=2.8e-11 PF21702: GLGE_C" amino acids 572 to 648 (77 residues), 66.4 bits, see alignment E=3.3e-22

Best Hits

KEGG orthology group: None (inferred from 96% identity to pfs:PFLU2761)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UHG8 at UniProt or InterPro

Protein Sequence (660 amino acids)

>PS417_12795 alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase (Pseudomonas simiae WCS417)
MTAEIESLLPLSQALLLPRIAIESTTPVIDGGEFAVKAVVGQQVNVTSKVFADGHDKLAV
LIRWRPLQDESWHSVVMTDVGNNGWEGTFTVTQQGPHEYCIEAWIDTFASFCYELRKKHE
AGVPVSLELQEGRSLVLQAAERSDNELRDRLMLLHHELSGLLETEQVAQFLHEDSAHLMT
QADHRAYLSISTVYPIDVEREAALFASWYELFPRSITDDPARHGTFNDVHSRLSMIHDMG
FDVLYFPPIHPIGRSHRKGKNNSLTAGPDDPGSPYAIGSEEGGHEAIHPQLGSRDDFRRL
VQAAAEHGLEIALDFAIQCSQDHPWLKAHPGWFNWRPDGTIKYAENPPKKYQDIVNVDFY
AADAIPSLWTELRDIVVGWVEEGVKTFRVDNPHTKPLPFWQWLISDVRSKYPEVIFLAEA
FTTPAMMARLGKVGYSQSYTYFTWRNTKAELSEYLTQLNESPWRECYRPNFFVNTPDINP
AFLHESGRAGFLIRAALATMGSGLWGMYSGFELCESAPVPGKEEYLDSEKYEIRPRDYTA
PGNIIAEIAQLNRIRRQNPALQTHLGLKLYNAWNDNILYFGKRSEDGSNFILVAVNLDPF
NAQEAHFELPLWELGLPDDAQTQGEDLMNGHRWTWYGKNQWTRLDPQMPFGIWRISHSPA