Protein Info for GFF250 in Pseudomonas sp. DMC3

Annotation: putative membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 transmembrane" amino acids 72 to 94 (23 residues), see Phobius details amino acids 100 to 125 (26 residues), see Phobius details amino acids 168 to 193 (26 residues), see Phobius details amino acids 253 to 278 (26 residues), see Phobius details PF11067: DUF2868" amino acids 144 to 440 (297 residues), 349.5 bits, see alignment E=1.1e-108

Best Hits

KEGG orthology group: None (inferred from 89% identity to pfo:Pfl01_5362)

Predicted SEED Role

"probable integral membrane protein NMA1899"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (457 amino acids)

>GFF250 putative membrane protein (Pseudomonas sp. DMC3)
VTELTPLQNLWLTETIRLREEHAGPLDDLEANRLARAAGGDLPSRIARRALWLAERDGLT
AALKHWLQGARLALVLLMIFAVLSGAGLAFAALGQTPVNVFWALGSLLGINLILLLSWAL
GLIFAGEHGATLGRLWLWLSGKFARDAKAAQLAPALLLLLQRKKLNRWALGALVNGLWLL
AMLSALILLLTLMATRRYGFVWETTILGADTFIHVTQALGYLPSLLGFNVPTEEMIRASG
AGALDIESARQAWATWLVGVLVVYGVLPRLLLALFCLWRWNSGNATLRLDLNLPGYAQLR
ERLMPTSERLGVNDPEPAQLHRIESNVGERASEGALLVAIELDEQRPWPPALPKNVSNAG
ILDSRESRHKLLEQLSRFPPARLAIACDPRRSPDRGSLALIAELARNAGATRIWLLQAPP
GEALDGQRLGDWHAALQQLELPFADCAPMNWLESGHD