Protein Info for GFF25 in Xanthobacter sp. DMC5

Annotation: Flavin reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 171 PF01613: Flavin_Reduct" amino acids 21 to 164 (144 residues), 151.6 bits, see alignment E=9.4e-49

Best Hits

Swiss-Prot: 39% identical to FRED_PSEPU: Flavin reductase from Pseudomonas putida

KEGG orthology group: None (inferred from 51% identity to put:PT7_3589)

MetaCyc: 37% identical to 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione 4-hydroxylase reductase component (Mycobacterium tuberculosis H37Rv)
RXN-12446 [EC: 1.5.1.36]

Predicted SEED Role

No annotation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.36

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (171 amino acids)

>GFF25 Flavin reductase (Xanthobacter sp. DMC5)
MSETKSAPSAVSATELRAFAGHFATGVAVVTTAHPDGALHGLTLNAVTSLSLDPPLYLIC
LDHRSNTLKALKESGHFAVHFLDRRQSEISRVFASKQEDKFAGVEHVVGESGSPLIGGVL
AAAECRLSEICTVGDHMIVIGEVQRTHVFGGEPLLYHRGGYTALEAERKVA