Protein Info for GFF2497 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Sensor protein basS/pmrB (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 transmembrane" amino acids 35 to 55 (21 residues), see Phobius details amino acids 183 to 206 (24 residues), see Phobius details PF08521: 2CSK_N" amino acids 40 to 183 (144 residues), 134.2 bits, see alignment E=5.4e-43 PF00512: HisKA" amino acids 261 to 324 (64 residues), 58.8 bits, see alignment E=6.7e-20 PF02518: HATPase_c" amino acids 374 to 471 (98 residues), 73 bits, see alignment E=4e-24

Best Hits

KEGG orthology group: K07649, two-component system, OmpR family, sensor histidine kinase TctE [EC: 2.7.13.3] (inferred from 67% identity to pol:Bpro_4688)

Predicted SEED Role

"Sensor protein basS/pmrB (EC 2.7.3.-)" in subsystem Lipid A modifications or Orphan regulatory proteins (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (494 amino acids)

>GFF2497 Sensor protein basS/pmrB (EC 2.7.3.-) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VIEAAPPPVAGRPIPKSFGLFQREQRSLFGEILDWMLTPLLLLWPLSLALTWFVAQGIAS
KPFDRALEFNLQALTQFVANQNGQISFNLNAQARDLLRADDSDLVYYQVIDPRGKLVSGE
LDFPRPREDETPEAGRVLLRDDVVRGDEVRVAYTWLERNDAQNLVLMQVAETRGKRSTLA
AEIIKGVMVPQFVILPLAVLLVWLALVRGIRPLNELEQRIRARKPDDLSPIEESAIPQEV
APLVSSINDLLTRLKASLTTQRRFLADAAHQLKTPLAGLRMQAELAQRESNPEEIHGSLK
QIARSSSRATHTVNQLLALARAETTGRTLPSARVDLARLVRGAVQDSVPRALEHGIDLGF
EGPEEVPEDCLMDGNPILLQEMVRNLVDNAINYSGRGGVVTARVLIDPFSGVQILQVEDN
GPGIPESEREFVMQPFYRALGTNVDGSGLGLTIVQEIAQQHGATVLIDSAHPDRHPPGLL
ASVRFTPKTPRQQA