Protein Info for GFF2495 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: DNA topoisomerase III, Burkholderia type (EC 5.99.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 980 TIGR01056: DNA topoisomerase III" amino acids 1 to 663 (663 residues), 535.1 bits, see alignment E=1.5e-164 PF01751: Toprim" amino acids 3 to 126 (124 residues), 30.1 bits, see alignment E=8.7e-11 PF01131: Topoisom_bac" amino acids 165 to 620 (456 residues), 364.1 bits, see alignment E=2e-112 PF13342: Toprim_Crpt" amino acids 678 to 741 (64 residues), 62.5 bits, see alignment 5.8e-21 amino acids 794 to 855 (62 residues), 54.8 bits, see alignment 1.5e-18 PF02201: SWIB" amino acids 911 to 979 (69 residues), 82.4 bits, see alignment 3.1e-27

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 80% identity to ajs:Ajs_3990)

Predicted SEED Role

"DNA topoisomerase III, Burkholderia type (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (980 amino acids)

>GFF2495 DNA topoisomerase III, Burkholderia type (EC 5.99.1.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKTLVIAEKPSVAQDIVRALTPVAGKFDKHDDHFENDKYVVTSAVGHLVEIAAPEQFDVK
RGKWSFAHLPVLPPYFELKPIDKTKSRLGAVVKLVKRKDVTRLINACDAGREGELIFRLI
EQYAGGLKNGSYQSLGKPVQRLWLQSMTPAAIRDGFEQLRSDEQMRGLADAARSRSEADW
MVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSIVVEREEKIRAHRSRDYWEIHASFLA
EAGEYPGKWFDPNFKKPTGDDADAELRADRVWSQREALAIAEAVRGKAASVKEESKPTTQ
ASGLLYDLTSLQREANGRFGFSAKTTLQLAQSLYERHKALTYPRTDSRALPEDYLPTVKQ
TFDMLAGSGMKHLAPHAKTAIDNGYIRPSKRIFDNSKVSDHFAIIPTLQAPSGLSEAEQK
LYDLVVRRFLAVFFPSAEFMVTTRITSSVGHHFKTEGKVLVKPGWMAVYGKEAAEDVVDA
KEGDKGQNLVPVREGETVRTETVEPKGLKTKPPARYSEATLLGAMEGAGKLVDDDELREA
MQEKGLGTPATRAAIIEGLLTEKYILREGREIIPTAKAFQLMTLLRGLGVEELSKPELTG
EWEYKLAQMEHGQLSRAAFMGEIAAMTERMVKKAKEYDRDTIPGNYATLSTPCPNCGGVV
KXNYRRYTCTGADGNSEGCGFSFGKSPAGRTFEPEEAEQLLRDKKIGPLEGFRSKAGWPF
VAEIVIKFNEEEKNWKLEFDFGDDKKGEESGELVDFTGSESLGACPKCGSPVHEHGSNYV
CEKSVPTLAQPTPSCDFKSGKIILQQPVAREQMSKLLDTGKTDLLDKFVSMRTRRAFKAF
LAWDKEAGKVNFEFEPRASKFPPRKTAAGAPAKAAPAKKAAPAKKAPAAKKAAAKTPKAP
RKTTAATGKQPSAALAAVIGDGLVSRPEVVKKLWDYIKAHGLQDATDKRRVNADAKLAAV
FGKPQVTMFEIAGIVGQHLS