Protein Info for PS417_12700 in Pseudomonas simiae WCS417

Updated annotation (from data): ABC transporter for D-Mannitol and D-Sorbitol, ATPase component
Rationale: Specific phenotype on D-Sorbitol; D-Mannitol. Also important for D-mannose utilization, but is closely related to a P. aeruginosa system that is apparently not a mannose transporter (the substrate-binding component PA2338 does not bind mannose, see PMC6829864).
Original annotation: ABC transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 PF00005: ABC_tran" amino acids 20 to 161 (142 residues), 118.2 bits, see alignment E=6.5e-38 PF17912: OB_MalK" amino acids 235 to 288 (54 residues), 34.8 bits, see alignment 3.9e-12 PF08402: TOBE_2" amino acids 281 to 352 (72 residues), 42.6 bits, see alignment E=8e-15

Best Hits

Swiss-Prot: 58% identical to MALK_YERPN: Maltose/maltodextrin import ATP-binding protein MalK (malK) from Yersinia pestis bv. Antiqua (strain Nepal516)

KEGG orthology group: K10230, sorbitol/mannitol transport system ATP-binding protein (inferred from 96% identity to pfs:PFLU2742)

MetaCyc: 86% identical to polyol ABC-type transporterATP-binding component MtlK (Pseudomonas fluorescens)
7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]

Predicted SEED Role

"Various polyols ABC transporter, ATP-binding component" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U984 at UniProt or InterPro

Protein Sequence (367 amino acids)

>PS417_12700 ABC transporter for D-Mannitol and D-Sorbitol, ATPase component (Pseudomonas simiae WCS417)
MANLKIKNLQKGFEGFSIIKGIDLEVNDKEFVVFVGPSGCGKSTLLRLIAGLEEVSEGTI
ELDGRDITEVTPAKRDLAMVFQTYALYPHMSVRKNMSFALDLAGVDKKLVESKVSEAARI
LELGPLLERKPKQLSGGQRQRVAIGRAIVRNPKIFLFDEPLSNLDAALRVQMRLELARLH
KELQATMIYVTHDQVEAMTLADKVVVLNSGRIEQVGSPLELYHQPANLFVAGFLGTPKMG
FLKGKVTRVESQSCEVQLDAGTLINLPLSGATLSVGSAVTLGIRPEHLEIASPGQTTLTV
TADVGERLGSDTFCHVITANGEPLTMRIRGDMASQYGETLHLHLDPAHCHLFDTDGVAVA
RPLRAAA