Protein Info for GFF2483 in Variovorax sp. SCN45

Annotation: Urease alpha subunit (EC 3.5.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 572 PF00449: Urease_alpha" amino acids 3 to 124 (122 residues), 179.1 bits, see alignment E=5.4e-57 TIGR01792: urease, alpha subunit" amino acids 4 to 572 (569 residues), 949.9 bits, see alignment E=2.7e-290 PF01979: Amidohydro_1" amino acids 130 to 452 (323 residues), 273.7 bits, see alignment E=3.9e-85

Best Hits

Swiss-Prot: 94% identical to URE1_VARPS: Urease subunit alpha (ureC) from Variovorax paradoxus (strain S110)

KEGG orthology group: K01428, urease subunit alpha [EC: 3.5.1.5] (inferred from 98% identity to vpe:Varpa_4858)

MetaCyc: 75% identical to urease alpha subunit (Sinorhizobium meliloti Rm2011)
Urease. [EC: 3.5.1.5]

Predicted SEED Role

"Urease alpha subunit (EC 3.5.1.5)" in subsystem Urea decomposition (EC 3.5.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.5

Use Curated BLAST to search for 3.5.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (572 amino acids)

>GFF2483 Urease alpha subunit (EC 3.5.1.5) (Variovorax sp. SCN45)
MATIGRRAYAEIFGPTVGDRVRLADTELLIEVEADYTLRAGGYGEEVKFGGGKTIRDGMA
QSQRTRAQGAVDTVLTNALILDHWGIVKADIGLKDGRIAAIGKAGNPDVQPGVDIVIGPG
TEIISCEGNIVTAGGIDSHIHFICPQQIEEALSSGVTTMLGGGTGPATGTFATTSTPGPW
HIERMLQAADAFPMNLGFLGKGNASLPHALQEQIEAGVIGLKLHEDWGTTPAAISNCMDV
ADATDTQVAIHSDTLNESGFVENTIAAVGGRAICAFHTEGAGGGHAPDILRVVGEANFLP
SSTNPTMPYTVNTLDEHVDMLMVCHHLDAGIPEDLAFAESRIRKETIAAEDVLHDLGAIS
MFSSDSQAMGRVGEVIIRTWQTAHKMKLQRGTLPEDSARNDNFRARRYVAKYTINPAIAH
GIAHEVGSLEVGKWADIVIWKPAFFGVKPFTILKGGTIAMAAMGDPNASIPTPQPVHYRP
MFGAYGGSLAKSSLTFVSQAGLASGIKERYGLAKHLSAVKNIRGVRKKDLIHNGYTPKME
IDAQTYAVRADGNLLTCEPAKLLPMAQRYFLF