Protein Info for GFF248 in Xanthobacter sp. DMC5

Annotation: Acetone carboxylase beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 717 PF05378: Hydant_A_N" amino acids 14 to 202 (189 residues), 132.1 bits, see alignment E=2.8e-42 PF01968: Hydantoinase_A" amino acids 227 to 513 (287 residues), 281.6 bits, see alignment E=1e-87 PF19278: Hydant_A_C" amino acids 526 to 717 (192 residues), 320.4 bits, see alignment E=4.8e-100

Best Hits

Swiss-Prot: 95% identical to ACXA_XANP2: Acetone carboxylase beta subunit (acxA) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K10855, acetone carboxylase, beta subunit [EC: 6.4.1.6] (inferred from 95% identity to xau:Xaut_3509)

MetaCyc: 95% identical to acetone carboxylase beta subunit (Xanthobacter autotrophicus Py2)
Acetone carboxylase. [EC: 6.4.1.6]

Predicted SEED Role

"Acetone carboxylase, beta subunit (EC 6.4.1.6) / N-methylhydantoinase A (EC 3.5.2.14)" in subsystem Hydantoin metabolism (EC 3.5.2.14, EC 6.4.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.14, 6.4.1.6

Use Curated BLAST to search for 3.5.2.14 or 6.4.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (717 amino acids)

>GFF248 Acetone carboxylase beta subunit (Xanthobacter sp. DMC5)
VNVPVGNLRSVQVLGIDAGGTMTDTFFVDQDGDFVVGKAQSTPQNEALGLIASSEDGLAN
WGMSLHEALAQLQTGVYSGTAMLNRVVQRKGLKCGLIVNRGMEDFHRMGRAVQSHLGYAY
EDRIHLNTHRYDPPLVPRQLTRGVIERTDMMGTQVIPLREESAREAARDLIAADVEGIVI
SLMHSYKNPVNERRVRDIVTEEVEKSGKKIPVFASADYYPVRKETHRTNTTILEGYAAEP
SRQTLSKISNAFKERGTKFDFRVMATHGGTISWKAKELARTIVSGPIGGVIGAKYLGEVL
GYKNIACSDIGGTSFDVALITQGEMTIKNDPDMARLVLSLPLVAMDSVGAGAGSFIRLDP
YTRAIKLGPDSAGYRVGVCWKESGIETVTISDCHMVLGYLNPDNFLGGAVKLDRQRSVDA
IKVQIADKLGLSVEDAAAGIIELLDSDLRDYLRSMISGKGYSPQSFVCFSYGGAGPVHTY
GYTEGLGFEDVIVPAWAAGFSAFGCAAADFEYRYDKSLDINMPPDAPEADKVKAVETLQA
AWEELTHNVLEEFKLNGYSADQVTLQPGYRMQYRGQLNDLEIESPMASAATAGDWDQLTD
AFNATYGRVYAASARSPELGYSVTGAIMRGMVPIPKPRIPKEPEEGETPPEEAKIGTRKF
YRKKKWVDAQLYKMEALRPGNRVMGPAIIESDATTFVVPDGFETWLDGHRLFHLKEV