Protein Info for GFF248 in Sphingobium sp. HT1-2

Annotation: MBL-fold metallo-hydrolase superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 PF00753: Lactamase_B" amino acids 41 to 211 (171 residues), 67.7 bits, see alignment E=1.4e-22 PF17778: BLACT_WH" amino acids 248 to 292 (45 residues), 35.3 bits, see alignment 9.4e-13

Best Hits

KEGG orthology group: None (inferred from 82% identity to sch:Sphch_1705)

Predicted SEED Role

"Metallo-beta-lactamase family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (295 amino acids)

>GFF248 MBL-fold metallo-hydrolase superfamily (Sphingobium sp. HT1-2)
MGAMATPFDPADLPTGILMTLSPLVARVLAPNPSPFTYTGTQTYLVGGADVAVIDPGPDE
AEHLDALVAAIGGRRVVAILCTHTHRDHSPAARPLSVMTGAPVIGCAPLTLSDDGPRADA
AFDADYRPDRVLGDGELVAGTGWTLAAVSTPGHTSNHLCFALAQDKALFTGDHVMGWSTS
VISPPDGDMTAYMRSMQLLLGRDDIVYYPAHGEPVENPQRLVRGMMGHRKQREGQIMRFL
ERNGDSAIPDMVAEMYKGVDPRLYGAAGRSVLAHLIDLNGRGMAAPLEDGRWQMR