Protein Info for GFF248 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: ATP-dependent DNA helicase RecQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 615 TIGR01389: ATP-dependent DNA helicase RecQ" amino acids 19 to 607 (589 residues), 906.4 bits, see alignment E=7.7e-277 TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 21 to 470 (450 residues), 682.6 bits, see alignment E=2.8e-209 PF00270: DEAD" amino acids 33 to 194 (162 residues), 89 bits, see alignment E=7.7e-29 PF00271: Helicase_C" amino acids 226 to 337 (112 residues), 81.4 bits, see alignment E=1.4e-26 PF16124: RecQ_Zn_bind" amino acids 348 to 410 (63 residues), 61.7 bits, see alignment E=2.2e-20 PF09382: RQC" amino acids 412 to 520 (109 residues), 116.4 bits, see alignment E=1.6e-37 PF00570: HRDC" amino acids 539 to 605 (67 residues), 87.4 bits, see alignment E=1.3e-28

Best Hits

Swiss-Prot: 100% identical to RECQ_SALTY: ATP-dependent DNA helicase RecQ (recQ) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 95% identity to ecs:ECs4752)

MetaCyc: 95% identical to ATP-dependent DNA helicase RecQ (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase RecQ" in subsystem DNA-replication or DNA repair, bacterial RecFOR pathway

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (615 amino acids)

>GFF248 ATP-dependent DNA helicase RecQ (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MVNGVNVAQAEVLNLESGAKQVLQETFGYQQFRPGQEAIIDTALSGRDCLVVMPTGGGKS
LCYQIPALLLDGLTVVVSPLISLMKDQVDQLLANGVAAACLNSTQSREQQLEVMAGCRTG
QIRLLYIAPERLMLDNFLDHLAHWNPVLLAVDEAHCISQWGHDFRPEYAALGQLRQRFPA
LPFMALTATADDTTRQDIIRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQ
RGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENAIRVDVQEKFQRDDLQIVVATV
AFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEE
KPAGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNCDICLDPPKQYDGLN
DAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDFGHDKLKVYGMGREKSHEHWVSVIR
QLIHLGLVMQNIAQHSALQLTDAARPVLRGDVPLKLAVPRIVALKPRVMQKSFGGNYDRK
LFAKLRKLRKAIADEENIPPYVVFNDATLIEMAEQMPVSASEMLSVNGVGMRKLERFGKE
FMALIRAHVDGDDEE