Protein Info for GFF2471 in Sphingobium sp. HT1-2

Annotation: Polyphosphate kinase (EC 2.7.4.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 712 TIGR03705: polyphosphate kinase 1" amino acids 16 to 688 (673 residues), 929.6 bits, see alignment E=5.6e-284 PF13089: PP_kinase_N" amino acids 17 to 121 (105 residues), 121.3 bits, see alignment E=5.8e-39 PF02503: PP_kinase" amino acids 135 to 306 (172 residues), 178.9 bits, see alignment E=2.2e-56 PF17941: PP_kinase_C_1" amino acids 335 to 499 (165 residues), 240 bits, see alignment E=2.7e-75 PF13090: PP_kinase_C" amino acids 506 to 674 (169 residues), 209.7 bits, see alignment E=5.5e-66

Best Hits

Swiss-Prot: 59% identical to PPK1_AZOC5: Polyphosphate kinase (ppk) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 92% identity to sch:Sphch_2822)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (712 amino acids)

>GFF2471 Polyphosphate kinase (EC 2.7.4.1) (Sphingobium sp. HT1-2)
VAEADPIASLSPAGERYFNRELSWLAFNQRVLEEAMNRAHPLLERLRFLSISGANLDEFF
SVRVAGLKGQQLQDVDLRSADGLTPAQQLGAITETTASLMRAQQKVWGALHGELVQVGIE
VIGPSAEMDVGCAGWLREHFLTQIFPILTPQALDPAHPFPFIPNQGLSIVFDLERLSDKQ
PIRELVMIPSSLARFVRIPGEPMRYMALEAVIRRFSADLFPGYRVRNSGVFRIIRDSDIE
IEEEAEDLVRYFRSAIKRRRRGRVIRMEIEERIPEPVEEMLQDMLQGHEAIVVEVEGFIG
IGDLSGIVDADRPDLKFEPYAPRFPERIREYGGDCFAAIRAKDIVVHHPYEAFDVVISFL
KQAASDPDVVAIKQTLYRAGKQSAIIRALIDAAEAGKSVTAVVELKARFDEEQNLMWADA
LERAGVQVVYGFIDWKTHAKVSMVVRREGDQFRSYCHFGTGNYHPITARIYTDLSFFTAD
PAYSRDAAALFNYITGYVEPQRLEKLVMSPRDLRDTLCACIDAEIDHVRAGRPGTIWAKM
NSLVDPAIIEKLYAASNAGVQIDLIVRGICCLRPGVPGMSENIRVKSVVGRFLEHSRITV
FGNGKALPNNGAKVYISSADWMPRNFDRRVEFLAPVENPTVHDQILDQVMVANLIDTEQS
WVLDRDGHYTRLEPGDRPFNLHRYFMTNPSLSGRGAAQEHGAVPTLRLRGRA