Protein Info for PS417_12585 in Pseudomonas simiae WCS417

Annotation: acriflavine resistance protein B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1030 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 339 to 358 (20 residues), see Phobius details amino acids 365 to 387 (23 residues), see Phobius details amino acids 393 to 412 (20 residues), see Phobius details amino acids 436 to 459 (24 residues), see Phobius details amino acids 472 to 496 (25 residues), see Phobius details amino acids 539 to 556 (18 residues), see Phobius details amino acids 867 to 886 (20 residues), see Phobius details amino acids 894 to 913 (20 residues), see Phobius details amino acids 919 to 940 (22 residues), see Phobius details amino acids 968 to 988 (21 residues), see Phobius details amino acids 998 to 1022 (25 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1029 (1029 residues), 1524.6 bits, see alignment E=0 PF00873: ACR_tran" amino acids 1 to 1021 (1021 residues), 1213.8 bits, see alignment E=0 PF03176: MMPL" amino acids 343 to 498 (156 residues), 35.6 bits, see alignment E=5.3e-13 amino acids 775 to 1020 (246 residues), 20.7 bits, see alignment E=1.9e-08

Best Hits

Swiss-Prot: 54% identical to MEXB_PSEAE: Multidrug resistance protein MexB (mexB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 63% identity to atu:Atu3202)

MetaCyc: 54% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UBF1 at UniProt or InterPro

Protein Sequence (1030 amino acids)

>PS417_12585 acriflavine resistance protein B (Pseudomonas simiae WCS417)
MPQFFIDRPIFAWVVALFILLAGLLAIPQLPVAQYPNVAPPKVEIYAVYPGASAQTLDES
VVSLIEQELNGADHLLYFESQSSLGSATITATFQPGTNPEMAQVDVQNRLKAVEPRLPQA
VTQQGLQVEKVSAGFLLLVTLTSNDGKLDDVALSDYLARNVMNELKRLDGVGKAQLYGAE
RAMRVWIDPQKLIGFNLTPADVNAAISAQNAQVSAGSIGDLPGTNTQEITAAILVKGQLS
TPAEFADIVLKANPDGSTVRIGDVARVEVGSQEYQFSTRLNGKPSTAVSVQLSPGANALN
TATLVRAKMDELSRYFPANVEYKIPYDTSPFVKVSITKVVYTLLEAMALVFAVMFLFLQN
VRYTLIPTLVVPIALMGTFATMLLLGFSINVLTMFGMVLAIGILVDDAIVVVENVERIMA
TEGLSPKEATKKAMGQITGAIIGITLVLVAVFLPMAFMAGSVGVIYQQFSLSMATSILFS
AFLALTLTPALCATLLKPIAKGEHHAKGGFFGWFNTRFEQLTDRYEGWVAYALKRSGRYL
LIYLVLLVGLGWMFSRLPSSFLPVEDQGYTITDIQLPPGASKNRTVQVAEQIEAHNAEEP
GVGDTTMIMGFSFSGSGQNAALAFTTLKDWSERSSDDSASSIADRANMAFSELKDAIAYA
VLPPPVDGLGTSSGFEFRLQDRGGVGHAALMAARTELLAAAEKSPILANVRESALAEAPQ
VQLEVDRKQANALGVSFADVGNVLSSAIGSAYINDFPNQGRMQRVVVQAEGDQRSQVADL
MKINVRNNAGKMVPLSAFVEAKWTQGPTQLTRYNGYPAIAISGEAAPGHSTGEAMDEIQR
LVSQLPAGLGQEWTGLSLQERLSGAQAPLLLGLSLLIVFLCLAALYESWSIPTSVLLVVP
LGVLGAVIAVGLRGMPNDVFFKVGLITIIGLSAKNAILIIEFAKDLYDQGEDLVTATLKA
ARLRLRPIIMTSLAFILGVVPLAIATGASSASQQAIGTGVIGGMITATLAVVFVPVFFVV
VMKLMKKKSR