Protein Info for GFF2466 in Pseudomonas sp. DMC3

Annotation: Flagellar biosynthetic protein FlhB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 transmembrane" amino acids 34 to 55 (22 residues), see Phobius details amino acids 88 to 119 (32 residues), see Phobius details amino acids 143 to 165 (23 residues), see Phobius details amino acids 185 to 212 (28 residues), see Phobius details TIGR00328: flagellar biosynthetic protein FlhB" amino acids 8 to 355 (348 residues), 407 bits, see alignment E=3.6e-126 PF01312: Bac_export_2" amino acids 8 to 348 (341 residues), 421.3 bits, see alignment E=1.5e-130

Best Hits

KEGG orthology group: K02401, flagellar biosynthetic protein FlhB (inferred from 89% identity to pfo:Pfl01_1552)

Predicted SEED Role

"Flagellar biosynthesis protein FlhB" in subsystem Flagellar motility or Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (379 amino acids)

>GFF2466 Flagellar biosynthetic protein FlhB (Pseudomonas sp. DMC3)
MAESESGQDKTEDPTEKRKKDSREKGEIARSKELNTLAVMMAGASALLIFGGMLAQEMMD
VMRLNFTLSREVVMDQGAMGRFLLESGLIALLAIQPVMITLLLAAIIGPISLGGWLFAAS
SLAPKFSRMNPAAGLKRMFSFKAVVELLKALAKFLITLGVALLVLSADVDDLLRIAHEPL
DMAIIHSVLLVGWSTLWLACGLIIIAAVDVPVQIWESMKKLKMTKQEVRDEHKDQEGRPE
VKQRIRQTQREMSQRRMMAAIPEADVVITNPTHYAVALKYDSEKGGAPVLLAKGSDFIAL
KIREIAVANNVMLLESPALARSIYYSTELEQEIPGGLYLAVAQVLAYVYQIRQHQAGKGK
RPDPLKDDLPIPPDLRRDS