Protein Info for PS417_12555 in Pseudomonas simiae WCS417

Updated annotation (from data): phosphogluconate 2-dehydrogenase KguD (EC 1.1.1.43)
Rationale: Variably important for glucose utilization, especially with nitrogen sources other than ammonia. 72% identical to the characterized KguD from P.putida (PP3376, see PMC4646247)
Original annotation: bifunctional glyoxylate/hydroxypyruvate reductase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 PF00389: 2-Hacid_dh" amino acids 5 to 319 (315 residues), 101.3 bits, see alignment E=5.5e-33 PF02826: 2-Hacid_dh_C" amino acids 108 to 287 (180 residues), 212.3 bits, see alignment E=5.5e-67 PF03446: NAD_binding_2" amino acids 148 to 258 (111 residues), 25 bits, see alignment E=2.5e-09

Best Hits

Swiss-Prot: 59% identical to GHRB_ESCF3: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)

KEGG orthology group: K00032, phosphogluconate 2-dehydrogenase [EC: 1.1.1.43] (inferred from 95% identity to pfs:PFLU2712)

MetaCyc: 82% identical to 2-dehydro-6-phosphogluconate reductase (Pseudomonas fluorescens)
Phosphogluconate 2-dehydrogenase. [EC: 1.1.1.43]

Predicted SEED Role

"2-ketogluconate 6-phosphate reductase (EC 1.1.1.43)" in subsystem 2-Ketogluconate Utilization (EC 1.1.1.43)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.43

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U644 at UniProt or InterPro

Protein Sequence (325 amino acids)

>PS417_12555 phosphogluconate 2-dehydrogenase KguD (EC 1.1.1.43) (Pseudomonas simiae WCS417)
MKKSVVLYKKLSAPLMARLHEQTDVTLIEALDDTGLAKLRDALPGAHGLLGASLRLDAKL
LDLAPQLEAVASVSVGVDNYDIDYLTARGILLSNTPDVLTETTADTGFALILATARRVVE
LADMVRAGQWNKNIGPAHFGSDVHGKTLGIIGMGRIGEALAQRGHFGFGMPVIYHSHSPK
PAVEARFGAQYRSLNDLLQQADFVCLTLPLTAETEKLIGAEQFARMGPETIFINISRGKV
VDEAALVEALQQRTIRAAGLDVFEKEPLDHSSPLLRLNNVVATPHIGSATHETREAMAKC
AVDNLLQALAGEKPNNLVNANAWNR