Protein Info for GFF2447 in Pseudomonas sp. DMC3

Annotation: Regulatory protein AtoC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 460 PF00072: Response_reg" amino acids 5 to 114 (110 residues), 97 bits, see alignment E=2.7e-31 PF00158: Sigma54_activat" amino acids 134 to 296 (163 residues), 237.3 bits, see alignment E=2.8e-74 PF14532: Sigma54_activ_2" amino acids 139 to 296 (158 residues), 73.6 bits, see alignment E=6.8e-24 PF07728: AAA_5" amino acids 154 to 272 (119 residues), 32.8 bits, see alignment E=2.3e-11 PF00004: AAA" amino acids 154 to 273 (120 residues), 23.4 bits, see alignment E=2.5e-08 PF02954: HTH_8" amino acids 406 to 445 (40 residues), 49.4 bits, see alignment 1.1e-16

Best Hits

KEGG orthology group: K10943, two component system, response regulator FlrC (inferred from 92% identity to pfo:Pfl01_1534)

Predicted SEED Role

"Flagellar regulatory protein FleQ" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (460 amino acids)

>GFF2447 Regulatory protein AtoC (Pseudomonas sp. DMC3)
MGIKVLLVEDDRSLREALADTLLLAGHDYHAVGSAEEALTAVAQQAFSLVVSDVNMPGMD
GHQLLALLRARQPQLPVLLMTAHGAVERAVDAMRQGAADYLVKPFEPKALLDLVARHALG
SIDAGEADGPVAVEPASAQLLELAARVARSDSTVLISGESGTGKEVLARYIHQQSRRASQ
PFIAINCAAIPDNMLEATLFGHEKGSFTGAIASQAGKFEQADGGTILLDEISEMPLSLQA
KLLRVLQEREVERVGARKPISLDIRVVATTNRDLAGEVAAGRFREDLFYRLSVFPLAWRP
LRERTADILPLAEKLLAKHVNKMKHAAAKLSPEAQACLTAYPWPGNVRELDNAIQRALIL
QQGGLIQPQDFCLSGAVTFAPLPIAASVIREVEIDSDSSGALGNDLRRREFQMIIDTLRA
ERGRRKEAAEKLGISPRTLRYKLAQMRDAGMDVEGYLFAT