Protein Info for GFF2442 in Variovorax sp. SCN45

Annotation: RND efflux system, membrane fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 35 to 368 (334 residues), 257.1 bits, see alignment E=1e-80 PF16576: HlyD_D23" amino acids 59 to 286 (228 residues), 54.8 bits, see alignment E=1.6e-18 PF13533: Biotin_lipoyl_2" amino acids 60 to 108 (49 residues), 31.8 bits, see alignment 1.9e-11 PF00529: CusB_dom_1" amino acids 66 to 354 (289 residues), 34.1 bits, see alignment E=4.2e-12 PF13437: HlyD_3" amino acids 171 to 283 (113 residues), 26 bits, see alignment E=2.6e-09

Best Hits

Swiss-Prot: 48% identical to ACRA_ECO57: Multidrug efflux pump subunit AcrA (acrA) from Escherichia coli O157:H7

KEGG orthology group: K03585, membrane fusion protein (inferred from 84% identity to vpe:Varpa_4879)

MetaCyc: 48% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"Membrane fusion protein of RND family multidrug efflux pump" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (383 amino acids)

>GFF2442 RND efflux system, membrane fusion protein (Variovorax sp. SCN45)
MTSKFLFPLSLLSVIVLAACGAKQPPAAETPPPEVGVVTLHASDVPVRTELAGRTAAFRV
SEVRPQVNGIVRKRLFEEGSMVRAGQVLYEIEPGPFEAAHEQARAALATSQASIASLRGK
ASRFGELIKANAVSRQEHEEAQAALVQAEAGVKAAQAAVKAARINLGFTRITAPIAGRIG
RSSVTEGALVVPGQTAALATIQRLDPIYVDVVQSSLELTRLKRRFISGDMAPASTQVGLR
MEDGLPYSHTGTLKFSEVAVDPATGSVTLRAEFPNPQGLLLPGMYVRAQVESGVERQAIL
APQRGVARNEKGEPTALVVGAGDKVELRALELRTTSGDQWIVSKGLREGDRLVVDGLQRA
HPGDKVKPVPVTAAPAATASSAD