Protein Info for PS417_12450 in Pseudomonas simiae WCS417

Annotation: glucose-6-phosphate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 507 TIGR00871: glucose-6-phosphate dehydrogenase" amino acids 16 to 505 (490 residues), 560.3 bits, see alignment E=1.8e-172 PF00479: G6PD_N" amino acids 20 to 205 (186 residues), 189.1 bits, see alignment E=1.3e-59 PF02781: G6PD_C" amino acids 208 to 504 (297 residues), 381.6 bits, see alignment E=2.3e-118

Best Hits

KEGG orthology group: K00036, glucose-6-phosphate 1-dehydrogenase [EC: 1.1.1.49] (inferred from 97% identity to pfs:PFLU2692)

Predicted SEED Role

"Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49)" in subsystem Entner-Doudoroff Pathway or Pentose phosphate pathway (EC 1.1.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.49

Use Curated BLAST to search for 1.1.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TZ18 at UniProt or InterPro

Protein Sequence (507 amino acids)

>PS417_12450 glucose-6-phosphate dehydrogenase (Pseudomonas simiae WCS417)
MTANGKKPKAEPAPPTTLFLFGAHGDLVKRLLMPALYNLSRDGLLGDGLRIVGVDHNAIS
DADFAKKLEDFIRTEAASKVKGSGENALDPERWAQLAKGISYVEGDFLDDSTYVDIGKKI
ADSGTGNAVFYLATAPRFFSDVVQRLGSAGLLTEIEDGFRRVVIEKPFGSDLATAEALNA
SLLKVMSEKQIYRIDHYLGKETVQNILISRFSNVLFEAFWNNHYIDHVQITAAETVGVET
RGNFFEKTGTLRDMVPNHLFQLLAMVAMEPPAAFGADAVRGEKAKVIGAVRPWSLEDARA
NSVRGQYTAGEIGGKSLPGYREEANVAPDSSTETFVALKVMIDNWRWVGVPFYLRTGKRM
SVRDTEIVICFKPAPYAQFRDTEVDELKPTYLKIQIQPNEGMWFDLLAKKPGPTLDMANI
QLGFAYKDFFEMQPSTGYETLIYDCMTGDQTLFQRADNIENGWRAVQPFLDAWKEDDGIQ
TYKAGEDGPAAADALLARDGRTWHSLG