Protein Info for GFF2415 in Variovorax sp. SCN45

Annotation: Trans-aconitate 2-methyltransferase (EC 2.1.1.144)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 254 PF13489: Methyltransf_23" amino acids 11 to 158 (148 residues), 60.6 bits, see alignment E=5.5e-20 PF05175: MTS" amino acids 22 to 99 (78 residues), 25.1 bits, see alignment E=4.3e-09 PF01728: FtsJ" amino acids 30 to 101 (72 residues), 29 bits, see alignment E=3.3e-10 PF13847: Methyltransf_31" amino acids 31 to 143 (113 residues), 61.2 bits, see alignment E=3.4e-20 PF08242: Methyltransf_12" amino acids 35 to 125 (91 residues), 54.2 bits, see alignment E=7.2e-18 PF08241: Methyltransf_11" amino acids 35 to 127 (93 residues), 57.9 bits, see alignment E=4.7e-19 PF13649: Methyltransf_25" amino acids 35 to 123 (89 residues), 63.4 bits, see alignment E=9.5e-21

Best Hits

Swiss-Prot: 56% identical to TAM_RHILO: Trans-aconitate 2-methyltransferase (tam) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K00598, trans-aconitate 2-methyltransferase [EC: 2.1.1.144] (inferred from 77% identity to del:DelCs14_3311)

MetaCyc: 40% identical to malonyl-[acyl-carrier protein] O-methyltransferase (Mycobacterium tuberculosis H37Rv)
RXN-11475 [EC: 2.1.1.197]

Predicted SEED Role

"Trans-aconitate 2-methyltransferase (EC 2.1.1.144)" (EC 2.1.1.144)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.144

Use Curated BLAST to search for 2.1.1.144 or 2.1.1.197

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (254 amino acids)

>GFF2415 Trans-aconitate 2-methyltransferase (EC 2.1.1.144) (Variovorax sp. SCN45)
MTWSAKQYSAFEEERTRPVRDLVAALPQREARTAVDLGCGPGNSTEVLAQRFPGARVSGM
DSSDDMIQAARKRMPGVNFDVADIATWQPAEPVDVILANASLQWLPDHATLYPRLARQLA
PGGSLAIQTPDNLEEPAHRLARETAKDPRWADRIGDVRHPARHGAIDYYEMLRPHCSRVD
VWRTTYYHPLAGGAAAVVEWFKGSALRPYLALLDAEQQAGFLASYLAVIAKAYPALKDGS
VLLPFPRLFIVATR