Protein Info for PS417_12310 in Pseudomonas simiae WCS417
Annotation: methionine synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 86% identical to METH_PSEAE: Methionine synthase (metH) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 65% identity to abo:ABO_1324)MetaCyc: 67% identical to cobalamin-dependent methionine synthase (Escherichia coli K-12 substr. MG1655)
Methionine synthase. [EC: 2.1.1.13]
Predicted SEED Role
"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)
MetaCyc Pathways
- aspartate superpathway (22/25 steps found)
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
- folate transformations III (E. coli) (9/9 steps found)
- folate transformations II (plants) (10/11 steps found)
- L-methionine biosynthesis III (4/4 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
- L-methionine salvage from L-homocysteine (2/3 steps found)
- folate transformations I (9/13 steps found)
- L-methionine biosynthesis I (3/5 steps found)
- superpathway of L-methionine salvage and degradation (8/16 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.1.1.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7UEP7 at UniProt or InterPro
Protein Sequence (1236 amino acids)
>PS417_12310 methionine synthase (Pseudomonas simiae WCS417) MSDRSVRLQALHQALKERILILDGGMGTMIQSYKLEENDYRGKRFADWPSDVKGNNDLLV ITRPDVIGGIEKAYLDAGADILETNTFNATRISMADYGMEALAFELNVEGARLARKIADA KTAETPDKPRFVAGVLGPTSRTCSLSPDVNNPGYRNVTFDELVENYTEATKGLIEGGADL ILIETIFDTLNAKAAIFAVQGVFEELNIELPIMISGTITDASGRTLSGQTTEAFWNSVAH AKPISVGLNCALGASELRPYLEELSNKANTHVSAHPNAGLPNEFGEYDELPSQTAKVIEE FAQSGFLNIVGGCCGTTPGHIEAIAKAVAGYAPRQIPDIPKACRLSGLEPFTIDRSSLFV NVGERTNITGSAKFARLIREDNYTEALEVALQQVEAGAQVIDINMDEGMLDSKKAMVTFL NLIAGEPDISRVPIMIDSSKWDVIEAGLKCIQGKGIVNSISMKEGVEQFIHHAKLCKRYG AAVVVMAFDEAGQADTEARKKEICKRSYDILVNEVGFPPEDIIFDPNIFAVATGIEEHNN YAVDFINACAYIRDELPYALTSGGVSNVSFSFRGNNPVREAIHSVFLLYAIRNGLTMGIV NAGQLEIYDQIPAELRDAVEDVVLNRTPDGTDALLAIADKYKGDGSVKEAETEEWRGWDV NKRLEHALVKGITTHIVEDTEESRLSFARPIEVIEGPLMSGMNIVGDLFGAGKMFLPQVV KSARVMKQAVAHLIPFIELEKGDKPEAKGKILMATVKGDVHDIGKNIVGVVLGCNGYDIV DLGVMVPAEKILQVAKEQKCDIIGLSGLITPSLDEMVHVAREMQRQDFHLPLMIGGATTS KAHTAVKIEPKYSNDAVVYVTDASRAVGVATQLLSKELKAGFVERTREEYVEVRERTSNR SARTERLSYPAAIAKKPQFDWSTYSPVVPTFTGAKVLDNIDLKVLAEYIDWTPFFISWDL AGKFPRILTDEVVGEAATALYADAQEMLKKLIDEKLISARAVFGFWPTNQVQDDDLEVYG DDGQPIAKLHHLRQQIIKTDGKPNFSLADFVAPKDSGVTDYIGGFITTAGIGAEEVAKAY QDAGDDYNSIMVKALADRLAEACAEWLHQQVRKEHWGYAKDEQLDNEALIKEQYSGIRPA PGYPACPDHTEKAQLFQLLDPEAREMHAGRSGVFLTEHYAMFPAAAVSGWYFAHPQAQYF AVGKVDKDQVASYTARKGQDLSVTERWLAPNLGYDN