Protein Info for GFF2397 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: D-amino acid dehydrogenase small subunit (EC 1.4.99.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 429 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details PF00070: Pyr_redox" amino acids 16 to 47 (32 residues), 24.4 bits, see alignment (E = 1.8e-08) PF12831: FAD_oxidored" amino acids 17 to 61 (45 residues), 29.1 bits, see alignment 3.3e-10 PF00890: FAD_binding_2" amino acids 17 to 124 (108 residues), 35.1 bits, see alignment E=4.5e-12 PF01134: GIDA" amino acids 17 to 47 (31 residues), 22.7 bits, see alignment (E = 2.4e-08) PF01266: DAO" amino acids 17 to 408 (392 residues), 233.1 bits, see alignment E=3.5e-72 PF13450: NAD_binding_8" amino acids 19 to 54 (36 residues), 36 bits, see alignment 3.4e-12

Best Hits

Swiss-Prot: 60% identical to DADA3_RHILO: D-amino acid dehydrogenase 3 (dadA3) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 74% identity to psa:PST_4110)

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (429 amino acids)

>GFF2397 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MLSLSDGFEREIHMAHLIVIGGGITGVTSAYALARQGHQVTLIEKHRYAGMETSHANGGQ
LSASNAEVWTHPSTLVKGLKWMFKADAPLLVNPRPSWHKLSWFAEFVASIPKYRNNTVAT
TRLAIAAREQLFGWAEHEGIDFDHKQRGILHIYRDRAGFEHAGEVSRLLAQGGLSRRAVT
PDEMRAIEPTLQGEYFGGWYTESDSTGDIHKFTHGLGLACARRGVRLMTAQAVSRVWSTG
DRARVMLANGEEVDADGVVICAGVRSRALGAQLGDRLNVYPVKGYSITVMLNDEQSQAAA
PQVSLLDDETKLVTSRLGLDRFRVAGTAEFNGYNLDIRADRIRPLVDWVNQCFPGVSTRR
VEPWAGLRPMLPDMMPRVGAGRHANVFYNTGHGHLGWTLSAITAHQLGEHVARWDMRRVP
RAVQVSNMV