Protein Info for PS417_12045 in Pseudomonas simiae WCS417

Annotation: iditol 2-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08240: ADH_N
  • amino acids 42 to 166 (125 residues), 79.5 bits, E=2e-26
• PF00107: ADH_zinc_N
  • amino acids 205 to 331 (127 residues), 78.9 bits, E=3.4e-26

Best Hits

Swiss-Prot: 53% identical to ELTD_MYCS2: Erythritol/L-threitol dehydrogenase (eltD) from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

KEGG orthology group: None (inferred from 97% identity to psb:Psyr_2567)

MetaCyc: 53% identical to erythritol/L-threitol dehydrogenase (Mycolicibacterium smegmatis)
1.1.1.M29 [EC: 1.1.1.M29]; 1.1.1.M28 [EC: 1.1.1.M29, 1.1.1.M28]; D-xylulose reductase. [EC: 1.1.1.M29, 1.1.1.M28, 1.1.1.9]; Ribitol 2-dehydrogenase. [EC: 1.1.1.M29, 1.1.1.M28, 1.1.1.9, 1.1.1.56]; L-arabinitol 4-dehydrogenase. [EC: 1.1.1.M29, 1.1.1.M28, 1.1.1.9, 1.1.1.56, 1.1.1.12]

Predicted SEED Role

"Sorbitol dehydrogenase (EC 1.1.1.14)" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization (EC 1.1.1.14)

MetaCyc Pathways

• D-sorbitol degradation I (3/3 steps found)
• xylitol degradation I (2/2 steps found)
• erythritol degradation II (2/3 steps found)
• ribitol degradation I (1/2 steps found)
• L-threitol degradation (2/4 steps found)
• L-arabinose degradation II (1/3 steps found)
• superpathway of pentose and pentitol degradation (16/42 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Pentose and glucuronate interconversions

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.14, 1.1.1.9

Use Curated BLAST to search for 1.1.1.12 or 1.1.1.14 or 1.1.1.56 or 1.1.1.9 or 1.1.1.M28 or 1.1.1.M29

Sequence Analysis Tools

See A0A1N7U5V6 at UniProt or InterPro

Protein Sequence (371 amino acids)

>PS417_12045 iditol 2-dehydrogenase (Pseudomonas simiae WCS417)
MSTVTERTPEQLSPVIPKTMQAVVCHGPEDYRLETVDVPVPGPDEILTKVELCGICMGDI
KTYRGAPSFWGDAEQPRYVKPPMIPGHEFVCRVVALGPGAEKRGVKVGDRVISEQIVPCW
GCRFCNHGQYWMCQKHDLYGFQNNVQGAMAQYMIFTKEGIIHKVPDSIAPDEAILIEPLA
CSLHAAERANVDFDDVVVVAGAGTLGLGIIGAVRMRNPKKLIVLDMKPERAALALRMGAD
EVWNPAEEDVLAKIREITDGYGCDIYIEATGHHKAVNQGLAMLRKLGRFVEFSVFNDEAT
VDWSIIGDRKELDVLGSHLGPYMYPRAIDFIGNRKIDMRDVVTHTFALADFKEAFAVMER
GDKSLKVVLQP