Protein Info for GFF236 in Xanthobacter sp. DMC5

Annotation: Biotin carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 1 to 444 (444 residues), 709.2 bits, E=1.1e-217
• PF00289: Biotin_carb_N
  • amino acids 1 to 110 (110 residues), 151.2 bits, E=4.1e-48
• PF02786: CPSase_L_D2
  • amino acids 115 to 321 (207 residues), 263.9 bits, E=2.9e-82
• PF02222: ATP-grasp
  • amino acids 137 to 291 (155 residues), 45.2 bits, E=2.6e-15
• PF07478: Dala_Dala_lig_C
  • amino acids 139 to 290 (152 residues), 43.9 bits, E=6.1e-15
• PF02785: Biotin_carb_C
  • amino acids 335 to 440 (106 residues), 125.7 bits, E=2.6e-40

Best Hits

Swiss-Prot: 62% identical to ACCC_PSEAE: Biotin carboxylase (accC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 95% identity to xau:Xaut_3502)

MetaCyc: 60% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• 3-hydroxypropanoate cycle (10/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (12/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• glyoxylate assimilation (8/13 steps found)
• jadomycin biosynthesis (3/9 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Protein Sequence (452 amino acids)

>GFF236 Biotin carboxylase (Xanthobacter sp. DMC5)
MFRKILIANRGEIALRILRACKELGIATVAVHSTADADAMHVRLADESVCIGPPPARESY
LNIPSLLAACEITGADAVHPGYGFLAENARFAEILADHNIHFIGPKAEHIRIMGDKIEAK
KTAQALGIPVVPGSDGGIHSDEEAHRVAAEIGFPVLVKAAAGGGGRGMKVAMTADDLSQA
LSTARTEAKAAFGDDAVYLEKYLSTPRHIEVQILGDGKGHAIHLGERDCSLQRRHQKVWE
EAPSPVITAAQRAEIGETCAAAMRKLGYIGVGTIEFLYENGNFYFIEMNTRIQVEHPVTE
MVTGIDLINEQIRVAAGEPLTLTQKDVVIEGHAIECRVNAEHPTTFRPSPGKITHYHVPG
GLGIRVDSNAYQGYVIPPTYDSLVGKLIVHGKTRDECLRRLRRALDEFVVDGIDTTLPLF
RTLVRTRDVAEGQYDIHWLEHFLAEGEGKNVG