Protein Info for GFF2349 in Xanthobacter sp. DMC5

Annotation: Bifunctional protein PaaZ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02278: phenylacetic acid degradation protein paaN
  • amino acids 5 to 676 (672 residues), 1057 bits, E=0
• PF00171: Aldedh
  • amino acids 41 to 445 (405 residues), 205.1 bits, E=1.6e-64
• PF01575: MaoC_dehydratas
  • amino acids 542 to 652 (111 residues), 91.4 bits, E=3.3e-30

Best Hits

Swiss-Prot: 53% identical to PAAZ_ECOLI: Bifunctional protein PaaZ (paaZ) from Escherichia coli (strain K12)

KEGG orthology group: K02618, phenylacetic acid degradation protein (inferred from 84% identity to xau:Xaut_0907)

MetaCyc: 53% identical to fused 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase and oxepin-CoA hydrolase (Escherichia coli K-12 substr. MG1655)
3-hydroxybutyryl-CoA dehydratase. [EC: 4.2.1.55]; RXNMETA-12671 [EC: 4.2.1.55, 3.3.2.12]; RXNMETA-12672 [EC: 4.2.1.55, 3.3.2.12, 1.2.1.91]

Predicted SEED Role

"Aldehyde dehydrogenase (EC 1.2.1.3), PaaZ" in subsystem Aromatic Amin Catabolism (EC 1.2.1.3)

MetaCyc Pathways

• phenylacetate degradation I (aerobic) (9/9 steps found)
• superpathway of phenylethylamine degradation (10/11 steps found)
• (2S)-ethylmalonyl-CoA biosynthesis (4/4 steps found)
• acetyl-CoA fermentation to butanoate (6/7 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• octane oxidation (4/5 steps found)
• L-tryptophan degradation X (mammalian, via tryptamine) (3/4 steps found)
• phytol degradation (3/4 steps found)
• ethanol degradation III (2/3 steps found)
• hypotaurine degradation (2/3 steps found)
• mitochondrial NADPH production (yeast) (3/5 steps found)
• superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (5/8 steps found)
• fatty acid α-oxidation I (plants) (2/4 steps found)
• putrescine degradation III (2/4 steps found)
• ethylmalonyl-CoA pathway (7/11 steps found)
• serotonin degradation (4/7 steps found)
• histamine degradation (1/3 steps found)
• 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
• noradrenaline and adrenaline degradation (8/13 steps found)
• dopamine degradation (2/5 steps found)
• aromatic biogenic amine degradation (bacteria) (3/8 steps found)
• ceramide degradation by α-oxidation (2/7 steps found)
• limonene degradation IV (anaerobic) (2/7 steps found)
• sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
• alkane oxidation (1/6 steps found)
• ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)

KEGG Metabolic Maps

• 1,2-Dichloroethane degradation
• 3-Chloroacrylic acid degradation
• Arginine and proline metabolism
• Ascorbate and aldarate metabolism
• Butanoate metabolism
• Fatty acid metabolism
• Glycerolipid metabolism
• Glycolysis / Gluconeogenesis
• Histidine metabolism
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Pyruvate metabolism
• Tryptophan metabolism
• Urea cycle and metabolism of amino groups
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.3

Use Curated BLAST to search for 1.2.1.3 or 1.2.1.91 or 3.3.2.12 or 4.2.1.55

Protein Sequence (677 amino acids)

>GFF2349 Bifunctional protein PaaZ (Xanthobacter sp. DMC5)
VTNILDSYALDRWFTPEAGLVDIPSAIDGRVVARASTRGLAFAAMVRHARDVGGPALRRL
TFHQRADMLKALGAHLTAHKDALYALAADTGATKRDNFFDIDGGIGTLYAYASRGRRELP
GERFVVEGAPEALSKNGTFIGLHVLTPLHGVAVHVNAFNFPCWGMLEKLAPAILAGMPVI
TKPATATAYVAEAVVKLIIDANILPAGALQFVAGSTGDLLDHLTGQDVLSFTGSAATSQA
LRDHPAVSRNAVRFIAERDSLNAAVLGPDAVEGTPEFDLFVAEVVREMTVKAGQKCTAIR
RIIVPKAQESAVIAALSARLSAIAVGDPRREDVRMGPLASRAQRTATQEAVKEIAAEAEI
VFGDPEKVEPLGADAATGAFMAPVLLRCVEPLKAKAPHEVEAFGPVATVMGYEAIADALA
LVAKGEGSLVASVYTYDPAVAEELVFGIAAHHGRLVLIDRDCAKESTGHGSPLPGLVHGG
PGRAGGGEEMGGLRGVFHYMQRTAIEGSPARLSALTRTWLSGAPAPVAPEHPFKRKFDAL
SVGDTVETASRTITLDDIEHFAHFTGDTFYAHMDEEAAKANPFFPGRVAHGYLILSFAAG
LFVDPAPGPLLANYGLDSLRFLKPVSPGDAIRVRLTVKNKQPARKPEYGEVRWDAEVFNQ
DGDTVARYELLTMSSRG