Protein Info for GFF2344 in Methylophilus sp. DMC18

Annotation: Glycerol-3-phosphate acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00023: acyl-phosphate glycerol 3-phosphate acyltransferase
  • amino acids 11 to 201 (191 residues), 194.2 bits, E=1e-61
• PF02660: G3P_acyltransf
  • amino acids 16 to 192 (177 residues), 179 bits, E=4e-57

Best Hits

Swiss-Prot: 60% identical to PLSY_METFK: Glycerol-3-phosphate acyltransferase (plsY) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K08591, glycerol-3-phosphate acyltransferase PlsY [EC: 2.3.1.15] (inferred from 70% identity to meh:M301_2495)

Predicted SEED Role

"Acyl-phosphate:glycerol-3-phosphate O-acyltransferase PlsY" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria

MetaCyc Pathways

• phosphatidylglycerol biosynthesis I (6/6 steps found)
• phosphatidylglycerol biosynthesis II (6/6 steps found)
• superpathway of phospholipid biosynthesis III (E. coli) (10/12 steps found)
• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• oleate biosynthesis III (cyanobacteria) (2/3 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• superpathway of cardiolipin biosynthesis (bacteria) (8/13 steps found)
• diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
• palmitoyl ethanolamide biosynthesis (2/6 steps found)
• superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
• stigma estolide biosynthesis (2/7 steps found)
• anandamide biosynthesis II (2/8 steps found)
• anandamide biosynthesis I (2/12 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15

Protein Sequence (203 amino acids)

>GFF2344 Glycerol-3-phosphate acyltransferase (Methylophilus sp. DMC18)
MNEIGFDIEALVWIAAAYLIGSIAFGIIISKLYRLPDPRTVGSGNIGATNVARSGKKSAA
ILTLLGDAFKGWLPTWLALQAGMLMDVVALVGLAVFIGHLYPIYHKFKGGKGVATALGVM
IAFSGWLALSVVAVWLAVFLISRISSLSALVAAVLAPLIAWQFLSPYPVYVAVVVVMSAF
LIIRHQSNIEKLLAGTESRFGKK